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  Utilization of mechanocatalytic oligosaccharides by ethanologenic Escherichia coli as a model microbial cell factory

Jin, T., Käldström, M., Benavides, A., Kaufman Rechulski, M. D., & Jarboe, L. R. (2020). Utilization of mechanocatalytic oligosaccharides by ethanologenic Escherichia coli as a model microbial cell factory. AMB Express, 10(1): 28. doi:10.1186/s13568-020-0965-4.

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 Creators:
Jin, Tao1, Author
Käldström, Mats2, Author           
Benavides, Adriana1, 3, Author
Kaufman Rechulski, Marcelo D.4, Author           
Jarboe, Laura R.1, Author
Affiliations:
1Department of Chemical and Biological Engineering, Iowa State University, 4134 Biorenewables Research Laboratory, 617 Bissell Rd, Ames, IA, USA, ou_persistent22              
2Research Group Rinaldi, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445617              
3BioMAP REU Program, Ames, IA, USA, ou_persistent22              
4Research Department Schüth, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445589              

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Free keywords: Biofuels; Fermentation; Coli; Mechanocatalytic; Oligosaccharides
 Abstract: Mechanocatalysis is a promising method for depolymerization of lignocellulosic biomass. Microbial utilization of the resulting oligosaccharides is one potential route of adding value to the depolymerized biomass. However, it is unclear how readily these oligosaccharides are utilized by standard cell factories. Here, we investigate utilization of cellulose subjected to mechanocatalytic depolymerization, using ethanologenic Escherichia coli as a model fermentation organism. The mechanocatalytic oligosaccharides supported ethanol titers similar to those observed when glucose was provided at comparable concentrations. Tracking of the various oligomers, using maltose (alpha-1,4) and cellobiose (beta-1,4) oligomers as representative standards of the orientation, but not linkage, of the glycosidic bond, suggests that the malto-like-oligomers are more readily utilized than cello-like-oligomers, consistent with poor growth with cellotetraose or cellopentaose as sole carbon source. Thus, mechanocatalytic oligosaccharides are a promising substrate for cell factories, and microbial utilization of these sugars could possibly be improved by addressing utilization of cello-like oligomers.

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Language(s): eng - English
 Dates: 2019-11-272020-01-282020-02-03
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1186/s13568-020-0965-4
 Degree: -

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Title: AMB Express
Source Genre: Journal
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Publ. Info: Heidelberg : Springer
Pages: - Volume / Issue: 10 (1) Sequence Number: 28 Start / End Page: - Identifier: ISSN: 2191-0855
CoNE: https://pure.mpg.de/cone/journals/resource/2191-0855