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  Optical recording with single cell resolution from monolayered slice cultures of rat hippocampus

Bonhoeffer, T., & Staiger, V. (1988). Optical recording with single cell resolution from monolayered slice cultures of rat hippocampus. Neuroscience Letters, 92(3), 259-264. doi:10.1016/0304-3940(88)90599-x.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-0C3E-9 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-0C3F-8
Genre: Journal Article

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Bonhoeffer, T1, 2, Author              
Staiger, V1, 2, Author              
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1Former Department Structure and Function of Natural Nerve-Net , Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497803              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              

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 Abstract: Voltage-sensitive dyes were used to optically record the membrane potentials from neurons in hippocampal slice cultures. Multi-channel recordings from these monolayered but otherwise 'organotypic' slice cultures had very good spatial as well as good temporal resolution (15 x 15 micron, 0.5 ms respectively). We show that in this preparation action potentials elicited by intracellular current pulses can be recorded optically and that single spikes are readily detectable without averaging. Furthermore, a new procedure which significantly reduces photodynamic damage is described. Our study demonstrates the feasibility of optical recording with single cell resolution in an organotypic mammalian CNS preparation.

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 Dates: 1988-10
 Publication Status: Published in print
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 Identifiers: DOI: 10.1016/0304-3940(88)90599-x
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Title: Neuroscience Letters
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 92 (3) Sequence Number: - Start / End Page: 259 - 264 Identifier: ISSN: 0304-3940
CoNE: https://pure.mpg.de/cone/journals/resource/954925512448