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  Molecular architecture of the SYCP3 fibre and its interaction with DNA

Bollschweiler, D., Radu, L., Joudeh, L., Plitzko, J. M., Henderson, R. M., Mela, I., et al. (2019). Molecular architecture of the SYCP3 fibre and its interaction with DNA. OPEN BIOLOGY, 9(10): 190094. doi:10.1098/rsob.190094.

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 Creators:
Bollschweiler, Daniel1, Author
Radu, Laura1, Author
Joudeh, Luay1, Author
Plitzko, Jürgen M.2, Author              
Henderson, Robert M.1, Author
Mela, Ioanna1, Author
Pellegrini, Luca1, Author
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1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: COMPLEX PROTEIN SYCP3; 3-DIMENSIONAL ORGANIZATION; CRYOELECTRON TOMOGRAPHY; SYNAPTONEMAL COMPLEXES; CHROMOSOME SYNAPSIS; MICROSCOPY REVEALS; AXIAL ELEMENTS; CORE; RECOMBINATION; COMPONENTmeiosis; homologous recombination; synaptonemal complex; SYCP3; cryoEM; AFM;
 Abstract: The synaptonemal complex (SC) keeps homologous chromosomes in close alignment during meiotic recombination. A hallmark of the SC is the presence of its constituent protein SYCP3 on the chromosome axis. During SC assembly, SYCP3 is deposited on both axes of the homologue pair, forming axial elements that fuse into the lateral element (LE) in the tripartite structure of the mature SC. We have used cryo-electron tomography and atomic force microscopy to study the mechanism of assembly and DNA binding of the SYCP3 fibre. We find that the three-dimensional architecture of the fibre is built on a highly irregular arrangement of SYCP3 molecules displaying very limited local geometry. Interaction between SYCP3 molecules is driven by the intrinsically disordered tails of the protein, with no contact between the helical cores, resulting in a flexible fibre assembly. We demonstrate that the SYCP3 fibre can engage in extensive interactions with DNA, indicative of an efficient mechanism for incorporation of DNA within the fibre. Our findings suggest that SYCP3 deposition on the chromosome axis might take place by polymerization into a fibre that is fastened to the chromosome surface via DNA binding.

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Language(s): eng - English
 Dates: 2019
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000521587300002
DOI: 10.1098/rsob.190094
 Degree: -

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Title: OPEN BIOLOGY
Source Genre: Journal
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Publ. Info: 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND : ROYAL SOC
Pages: - Volume / Issue: 9 (10) Sequence Number: 190094 Start / End Page: - Identifier: -