English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A helical inner scaffold provides a structural basis for centriole cohesion

Le Guennec, M., Klena, N., Gambarotto, D., Laporte, M. H., Tassin, A.-M., Van den Hoek, H., et al. (2020). A helical inner scaffold provides a structural basis for centriole cohesion. SCIENCE ADVANCES, 6(7): eaaz4137. doi:10.1126/sciadv.aaz4137.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
eaaz4137.full.pdf (Any fulltext), 3MB
Name:
eaaz4137.full.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
open access article
License:
-

Locators

show

Creators

show
hide
 Creators:
Le Guennec, Maeva1, Author
Klena, Nikolai1, Author
Gambarotto, Davide1, Author
Laporte, Marine H.1, Author
Tassin, Anne-Marie1, Author
Van den Hoek, Hugo2, Author           
Erdmann, Philipp S.2, Author           
Schaffer, Miroslava2, Author           
Kovacik, Lubomir1, Author
Borgers, Susanne1, Author
Goldie, Kenneth N.1, Author
Stahlberg, Henning1, Author
Bornens, Michel1, Author
Azimzadeh, Juliette1, Author
Engel, Benjamin D.2, Author           
Hamel, Virginie1, Author
Guichard, Paul1, Author
Affiliations:
1external, ou_persistent22              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

Content

show
hide
Free keywords: BASAL BODIES; DE-NOVO; PROTEIN; ARCHITECTURE; VISUALIZATION; PURIFICATION; CENTROSOMES; DISRUPTION; FAM161A; POC1
 Abstract: The ninefold radial arrangement of microtubule triplets (MTTs) is the hallmark of the centriole, a conserved organelle crucial for the formation of centrosomes and cilia. Although strong cohesion between MTTs is critical to resist forces applied by ciliary beating and the mitotic spindle, how the centriole maintains its structural integrity is not known. Using cryo-electron tomography and subtomogram averaging of centrioles from four evolutionarily distant species, we found that MTTs are bound together by a helical inner scaffold covering similar to 70% of the centriole length that maintains MTTs cohesion under compressive forces. Ultrastructure Expansion Microscopy (U-ExM) indicated that POC5, POC1B, FAM161A, and Centrin-2 localize to the scaffold structure along the inner wall of the centriole MTTs. Moreover, we established that these four proteins interact with each other to form a complex that binds microtubules. Together, our results provide a structural and molecular basis for centriole cohesion and geometry.

Details

show
hide
Language(s): eng - English
 Dates: 2020
 Publication Status: Published online
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000518996500038
DOI: 10.1126/sciadv.aaz4137
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: SCIENCE ADVANCES
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA : AMER ASSOC ADVANCEMENT SCIENCE
Pages: - Volume / Issue: 6 (7) Sequence Number: eaaz4137 Start / End Page: - Identifier: ISSN: 2375-2548