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  Turning cell adhesions ON or OFF with high spatiotemporal precision using the green light responsive protein CarH

Xu, D., Ricken, J., & Wegner, S. (2020). Turning cell adhesions ON or OFF with high spatiotemporal precision using the green light responsive protein CarH. Chemistry – A European Journal, 1-5. doi:10.1002/chem.202001238.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-11D4-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-11D5-6
Genre: Journal Article

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Chemistry_epub_2020_238.pdf (Any fulltext), 2MB
 
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Chemistry_epub_2020_238_Suppl.pdf (Supplementary material), 458KB
 
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 Creators:
Xu, Dongdong, Author
Ricken, Julia1, Author              
Wegner, Seraphine, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: CarH; Cell adhesion; green light; photoregulation; spatiotemporal control
 Abstract: Spatiotemporal control of integrin-mediated cell adhesions to extracellular matrix regulates cell behavior with has numerous implications for biotechnological applications. Herein, we report two approaches for regulating cell adhesions in space and time with high precision both of which utilize green light. In the first design, CarH, which is a tetramer in the dark, is used to mask cRGD adhesion-peptides on a surface. Upon green light illumination, the CarH tetramer dissociates into its monomers, revealing the adhesion peptide so that cells can adhere. In the second design, the RGD motif is incorporated into the CarH protein tetramer such that cells can adhere to surfaces functionalized with this protein. The cell adhesions can be disrupted with green light, due to the disassembly of the CarH-RGD protein. Both designs allow for photoregulation with noninvasive visible light and open new possibilities to investigate the dynamical regulation of cell adhesions in cell biology.

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Language(s): eng - English
 Dates: 2020-04-09
 Publication Status: Published online
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Chemistry – A European Journal
  Other : Chem. – Eur. J.
  Other : Chem. Eur. J.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 1 - 5 Identifier: ISSN: 0947-6539
CoNE: https://pure.mpg.de/cone/journals/resource/954926979058