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  Metabolic reprogramming of fibro/adipogenic progenitors facilitates muscle regeneration

Reggio, A., Rosina, M., Krahmer, N., Palma, A., Petrilli, L. L., Maiolatesi, G., et al. (2020). Metabolic reprogramming of fibro/adipogenic progenitors facilitates muscle regeneration. LIFE SCIENCE ALLIANCE, 3(3): e202000646. doi:10.26508/lsa.202000660.

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 Creators:
Reggio, Alessio1, Author
Rosina, Marco1, Author
Krahmer, Natalie2, Author           
Palma, Alessandro1, Author
Petrilli, Lucia Lisa1, Author
Maiolatesi, Giuliano1, Author
Massacci, Giorgia1, Author
Salvatori, Illari1, Author
Valle, Cristiana1, Author
Testa, Stefano1, Author
Gargioli, Cesare1, Author
Fuoco, Claudia1, Author
Castagnoli, Luisa1, Author
Cesareni, Gianni1, Author
Sacco, Francesca1, Author
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1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: DUCHENNE MUSCULAR-DYSTROPHY; SKELETAL-MUSCLE; SATELLITE CELLS; CALORIE RESTRICTION; GENE-EXPRESSION; MOUSE MODEL; STEM-CELLS; FIBROSIS; INJURY; SWITCH
 Abstract: In Duchenne muscular dystrophy (DMD), the absence of the dystrophin protein causes a variety of poorly understood secondary effects. Notably, muscle fibers of dystrophic individuals are characterized by mitochondrial dysfunctions, as revealed by a reduced ATP production rate and by defective oxidative phosphorylation. Here, we show that in a mouse model of DMD (mdx), fibro/adipogenic progenitors (FAPs) are characterized by a dysfunctional mitochondrial metabolism which correlates with increased adipogenic potential. Using high-sensitivity mass spectrometry-based proteomics, we report that a short-term high-fat diet (HFD) reprograms dystrophic FAP metabolism in vivo. By combining our proteomic dataset with a literature-derived signaling network, we revealed that HFD modulates the beta-catenin-follistatin axis. These changes are accompanied by significant amelioration of the histological phenotype in dystrophic mice. Transplantation of purified FAPs from HFD-fed mice into the muscles of dystrophic recipients demonstrates that modulation of FAP metabolism can be functional to ameliorate the dystrophic phenotype. Our study supports metabolic reprogramming of muscle interstitial progenitor cells as a novel approach to alleviate some of the adverse outcomes of DMD.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published online
 Pages: 24
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000523303300009
DOI: 10.26508/lsa.202000660
 Degree: -

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Title: LIFE SCIENCE ALLIANCE
Source Genre: Journal
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Publ. Info: 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USA : LIFE SCIENCE ALLIANCE LLC
Pages: - Volume / Issue: 3 (3) Sequence Number: e202000646 Start / End Page: - Identifier: -