ausblenden:
Schlagwörter:
COMPATIBLE SOLUTE ACCUMULATION; TRAP-TRANSPORTER TEAABC;
ESCHERICHIA-COLI; GLYCINE BETAINE; CORYNEBACTERIUM-GLUTAMICUM;
BACILLUS-SUBTILIS; BACTERIA DOMAIN; PROTEIN; OSMOREGULATION; STRESSHalomonas elongata; Ectoine; Mechanosensitive channel; MscS; MscK;
Osmoadaptation; Solute excretion; Osmotic shock;
Zusammenfassung:
For osmoadaptation the halophilic bacterium Halomonas elongata synthesizes as its main compatible solute the aspartate derivative ectoine. H. elongata does not rely entirely on synthesis but can accumulate ectoine by uptake from the surrounding environment with the help of the osmoregulated transporter TeaABC. Disruption of the TeaABC-mediated ectoine uptake creates a strain that is constantly losing ectoine to the medium. However, the efflux mechanism of ectoine in H. elongata is not yet understood. H. elongata possesses four genes encoding mechanosensitive channels all of which belong to the small conductance type (MscS). Analysis by qRT-PCR revealed a reduction in transcription of the mscS genes with increasing salinity. The response of H. elongata to hypo- and hyperosmotic shock never resulted in up-regulation but rather in down-regulation of mscS transcription. Deletion of all four mscS genes created a mutant that was unable to cope with hypoosmotic shock. However, the knockout mutant grew significantly faster than the wildtype at high salinity of 2 M NaCl, and most importantly, still exported 80% of the ectoine compared to the wildtype. We thus conclude that a yet unknown system, which is independent of mechanosensitive channels, is the major export route for ectoine in H. elongata.
