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  Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function

Dodonova, S. O., Zhu, F., Dienemann, C., Taipale, J., & Cramer, P. (2020). Nucleosome-bound SOX2 and SOX11 structures elucidate pioneer factor function. Nature, 580(7805), 669-672. doi:10.1038/s41586-020-2195-y.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-55D5-A Version Permalink: http://hdl.handle.net/21.11116/0000-0006-55DB-4
Genre: Journal Article

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Dodonova, S. O.1, Author              
Zhu, Fangjie, Author
Dienemann, C.1, Author              
Taipale, J., Author
Cramer, P.1, Author              
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1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: Cryoelectron microscopy; Nucleosomes; Transcription
 Abstract: 'Pioneer’ transcription factors are required for stem-cell pluripotency, cell differentiation and cell reprogramming1,2. Pioneer factors can bind nucleosomal DNA to enable gene expression from regions of the genome with closed chromatin. SOX2 is a prominent pioneer factor that is essential for pluripotency and self-renewal of embryonic stem cells3. Here we report cryo-electron microscopy structures of the DNA-binding domains of SOX2 and its close homologue SOX11 bound to nucleosomes. The structures show that SOX factors can bind and locally distort DNA at superhelical location 2. The factors also facilitate detachment of terminal nucleosomal DNA from the histone octamer, which increases DNA accessibility. SOX-factor binding to the nucleosome can also lead to a repositioning of the N-terminal tail of histone H4 that includes residue lysine 16. We speculate that this repositioning is incompatible with higher-order nucleosome stacking, which involves contacts of the H4 tail with a neighbouring nucleosome. Our results indicate that pioneer transcription factors can use binding energy to initiate chromatin opening, and thereby facilitate nucleosome remodelling and subsequent transcription.

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Language(s): eng - English
 Dates: 2020-04-222020-04-30
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41586-020-2195-y
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Title: Nature
Source Genre: Journal
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Pages: - Volume / Issue: 580 (7805) Sequence Number: - Start / End Page: 669 - 672 Identifier: -