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  Osteogenic and chondrogenic potential of the supramolecular aggregate T-LysYal®

Benedetto, A. D., Posa, F., Marazzi, M., Kalemaj, Z., Grassi, R., Muzio, L. L., et al. (2020). Osteogenic and chondrogenic potential of the supramolecular aggregate T-LysYal®. Frontiers in Endocrinology, 11: 285, pp. 1-11. doi:10.3389/fendo.2020.00285.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-5A78-F Version Permalink: http://hdl.handle.net/21.11116/0000-0006-5A79-E
Genre: Journal Article

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 Creators:
Benedetto, Adriana Di, Author
Posa, Francesca1, 2, Author              
Marazzi, Mario, Author
Kalemaj, Zamira, Author
Grassi, Roberta, Author
Muzio, Lorenzo Lo, Author
Comite, Mariasevera Di, Author
Cavalcanti-Adam, Elisabetta Ada3, Author              
Grassi, Felice Roberto, Author
Mori, Giorgio, Author
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              
3Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: bone, cartilage, supramolecular aggregate, mesenchymal stem cells, tissue regeneration, focal adhesions
 Abstract: Hard tissue regeneration represents a challenge for the Regenerative Medicine and Mesenchymal stem cells (MSCs) could be a successful therapeutic strategy. T-LysYal® (T-Lys), a new derivative of Hyaluronic Acid (HA) possessing a superior stability, has already been proved efficient in repairing corneal epithelial cells damaged by dry conditions in vitro. We investigated the regenerative potential of T-Lys in the hard tissues bone and cartilage. We have previously demonstrated that cells isolated from the tooth germ, Dental Bud Stem Cells (DBSCs), differentiate into osteoblast-like cells, representing a promising source of MSCs for bone regeneration. Herewith, we show that T-Lys treatment stimulates the expression of typical osteoblastic markers, such as Runx-2, Collagen I (Col1) and Alkaline Phosphatase (ALP), determining a higher production of mineralized matrix nodules. In addition, we found that T-Lys treatment positively affects αVβ3 integrin expression, key integrin in the osteoblastic commitment, leading to the formation of focal adhesions (FAs). The efficacy of T-Lys was also tested on chondrogenic differentiation starting from human articular chondrocytes (HACs) resulting in an increase of differentiation markers and cell number.

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Language(s): eng - English
 Dates: 2020-01-282020-04-162020-05-05
 Publication Status: Published online
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.3389/fendo.2020.00285
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Title: Frontiers in Endocrinology
  Abbreviation : Front. Endocrinol.
Source Genre: Journal
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Publ. Info: Lausanne : Frontiers
Pages: - Volume / Issue: 11 Sequence Number: 285 Start / End Page: 1 - 11 Identifier: ISSN: 1664-2392
CoNE: https://pure.mpg.de/cone/journals/resource/1664-2392