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  Exploiting natural variation in tomato to define pathway structure and metabolic regulation of fruit polyphenolics in the lycopersicum complex

Tohge, T., Scossa, F., Wendenburg, R., Frasse, P., Balbo, I., Watanabe, M., et al. (2020). Exploiting natural variation in tomato to define pathway structure and metabolic regulation of fruit polyphenolics in the lycopersicum complex. Molecular Plant, 13(7), 1027-1046. doi:10.1016/j.molp.2020.04.004.

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 Creators:
Tohge, T.1, Author              
Scossa, F.1, Author              
Wendenburg, R.1, Author              
Frasse, Pierre2, Author
Balbo, I.1, Author              
Watanabe, M.3, Author              
Alseekh, S.1, Author              
Jadha, Sagar Sudam2, Author
Delfin, Jay C.2, Author
Lohse, M.4, Author              
Giavalisco, Patrick2, Author
Usadel, B.4, Author              
Zhang, YJ1, Author              
Luo, Jie2, Author
Bouzayen, Mondher2, Author
Fernie, A. R.1, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2external, ou_persistent22              
3Amino Acid and Sulfur Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753337              
4Integrative Carbon Biology, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753329              

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Free keywords: , secondary metabolism, natural diversity, wild accessions, pathway elucidation, gene discovery
 Abstract: Whilst the structures of plant primary metabolic pathways are generally well defined and highly conserved across species, those defining specialized metabolism are less well characterized and more highly variable across species. Here, we investigate polyphenolic metabolism in the lycopersicum complex by characterizing the underlying biosynthetic and decorative reactions which comprise the metabolic network of polyphenols across eight different species of tomato. For this purpose, GC- and LC-MS based metabolomics were carried out, in concert with the evaluation of cross-hybridized-microarray for MapMan based-transcriptomic analysis, and publically available RNA sequencing data for annotation of biosynthetic genes respectively, in different tissues of Solanum lycopersicum and wild tomato species. The combined data was used to compile species-specific metabolic networks of polyphenolic metabolism allowing the proposal of an entire pan-species biosynthetic framework as well as to annotate functions of decoration enzymes involved in the creation of metabolic diversity of the flavonoid pathway. The combined results are discussed both in the context of current understanding of tomato flavonol biosynthesis as well as a global view of metabolic shift during fruit ripening. Our results are providing an example as to how large-scale biology approaches can be used for the definition and refinement of large pathways of specialized metabolism.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
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Title: Molecular Plant
Source Genre: Journal
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Publ. Info: Oxford University Press
Pages: - Volume / Issue: 13 (7) Sequence Number: - Start / End Page: 1027 - 1046 Identifier: ISSN: 1674-2052
CoNE: https://pure.mpg.de/cone/journals/resource/1674-2052