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  Functional neuroanatomical mapping in insects by [3H]2-deoxy-D-glucose at electron microscopical resolution

Buchner, S., & Buchner, E. (1982). Functional neuroanatomical mapping in insects by [3H]2-deoxy-D-glucose at electron microscopical resolution. Neuroscience Letters, 28(3), 235-240. doi:10.1016/0304-3940(82)90063-5.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-67B9-6 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-67BB-4
Genre: Journal Article

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Buchner, S1, 2, Author              
Buchner, E1, 2, Author              
Affiliations:
1Former Department Neurophysiology of Insect Behavior, Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497802              
2Max Planck Institute for Biological Cybernetics, Max Planck Society, ou_1497794              

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 Abstract: Electron microscope autoradiography of insect nervous tissue prepared for ‘activity staining’ by the deoxyglucose method provides information on metabolic activity of small identifiable nerve fibers. Grain counts over two sets of axon profiles selected for high and low radioactivity, respectively, indicate that the dynamic range of the present method comprises about one order of magnitude. It is demonstrated that, although a small fraction of the radioactive label is lost during flotation of the thin sections on a water surface, the distribution of the remaining label is not appreciably altered and reflects stimulus-induced nervous activity.

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 Dates: 1982-03
 Publication Status: Published in print
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 Identifiers: DOI: 10.1016/0304-3940(82)90063-5
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Title: Neuroscience Letters
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 28 (3) Sequence Number: - Start / End Page: 235 - 240 Identifier: ISSN: 0304-3940
CoNE: https://pure.mpg.de/cone/journals/resource/954925512448