English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A glycan array-based assay for the identification and characterization of plant glycosyltransferases

Ruprecht, C., Bartetzko, M. P., Senf, D., Lakhina, A., Smith, P. J., Soto, M. J., et al. (2020). A glycan array-based assay for the identification and characterization of plant glycosyltransferases. Angewandte Chemie International Edition. doi:10.1002/anie.202003105.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0006-6C83-D Version Permalink: http://hdl.handle.net/21.11116/0000-0006-6C84-C
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Ruprecht, Colin1, Author              
Bartetzko, Max Peter1, Author              
Senf, Deborah1, Author              
Lakhina, Anna, Author
Smith, Peter J., Author
Soto, Maria J., Author
Oh, Hyunil, Author
Yang, Jeong-Yeh, Author
Chapla, Digantkumar, Author
Varón Silva, Daniel2, Author              
Clausen, Mads H., Author
Hahn, Michael G., Author
Moremen, Kelley W., Author
Urbanowicz, Breeanna R., Author
Pfrengle, Fabian1, Author              
Affiliations:
1Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863303              
2Daniel Varón Silva, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863302              

Content

show
hide
Free keywords: carbohydrates, Plant Cell Wall, Glycan Array, Glycosyltransferases, Sugar Nucleotides
 Abstract: Growing plants with modified cell wall compositions is a promising strategy to improve resistance to pathogens, increase biomass digestibility, and tune other important properties. In order to alter biomass architecture, a detailed knowledge of cell wall structure and biosynthesis is a prerequisite. We report here a glycan array-based assay for the high-throughput identification and characterization of plant cell wall biosynthetic glycosyltransferases (GTs). We demonstrate that different heterologously expressed galactosyl-, fucosyl-, and xylosyltransferases can transfer azido-functionalized sugar nucleotide donors to selected synthetic plant cell wall oligosaccharides on the array and that the transferred monosaccharides can be visualized “on chip” by a 1,3-dipolar cycloaddition reaction with an alkynyl-modified dye. The opportunity to simultaneously screen thousands of combinations of putative GTs, nucleotide sugar donors, and oligosaccharide acceptors will dramatically accelerate plant cell wall biosynthesis research.

Details

show
hide
Language(s): eng - English
 Dates: 2020-05-12
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: DOI: 10.1002/anie.202003105
DOI: 10.1002/ange.202003105
BibTex Citekey: doi:10.1002/anie.202003105
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Angewandte Chemie International Edition
  Abbreviation : Angew. Chem., Int. Ed.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1433-7851

Source 2

show
hide
Title: Angewandte Chemie
  Abbreviation : Angew. Chem.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 0044-8249