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  Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats

Klioutchnikov, A., Wallace, D. J., Frosz, M. H., Zeltner, R., Sawinski, J., Pawlak, V., et al. (2020). Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats. Nature methods, 17, 509-513. doi:10.1038/s41592-020-0817-9.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0006-6CED-7 Version Permalink: https://hdl.handle.net/21.11116/0000-0006-6CEE-6
Genre: Journal Article

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 Creators:
Klioutchnikov, Alexandr1, Author
Wallace, Damian James1, Author
Frosz, Michael H.2, 3, Author           
Zeltner, Richard3, Author           
Sawinski, Jürgen1, Author
Pawlak, Verena1, Author
Voit, Kay-Michael1, Author
Russell, Philip St. J.3, Author           
Kerr, Jason1, Author
Affiliations:
1Center of Advanced European Studies and Research (caesar), ou_persistent22              
2Fibre Fabrication and Glass Studio, Technology Development and Service Units, Max Planck Institute for the Science of Light, Max Planck Society, ou_2364724              
3Russell Division, Max Planck Institute for the Science of Light, Max Planck Society, ou_2364721              

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 Abstract: We designed a head-mounted three-photon microscope for imaging deep cortical layer neuronal activity in a freely moving rat. Delivery of high-energy excitation pulses at 1,320 nm required both a hollow-core fiber whose transmission properties did not change with fiber movement and dispersion compensation. These developments enabled imaging at >1.1 mm below the cortical surface and stable imaging of layer 5 euronal activity for >1 h in freely moving rats performing a range of behaviors.

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Language(s): eng - English
 Dates: 2020-05-04
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41592-020-0817-9
 Degree: -

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Title: Nature methods
  Abbreviation : Nat methods
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 17 Sequence Number: - Start / End Page: 509 - 513 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556