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  Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

Faizi, H. A., Reeves, C. J., Georgiev, V., Vlahovska, P. M., & Dimova, R. (2020). Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy. Soft Matter, 16(39), 8996-9001. doi:10.1039/D0SM00943A.

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Faizi, Hammad A., Author
Reeves, Cody J., Author
Georgiev, Vasil1, Author              
Vlahovska, Petia M., Author
Dimova, Rumiana1, Author              
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1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              

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 Abstract: A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.

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Language(s): eng - English
 Dates: 2020-09-172020
 Publication Status: Published in print
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 Identifiers: DOI: 10.1039/D0SM00943A
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Title: Soft Matter
  Abbreviation : Soft Matter
Source Genre: Journal
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Publ. Info: Cambridge, UK : Royal Society of Chemistry
Pages: - Volume / Issue: 16 (39) Sequence Number: - Start / End Page: 8996 - 9001 Identifier: ISSN: 1744-683X