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  Illumination guidelines for ultrafast pump–probe experiments by serial femtosecond crystallography

Grünbein, M. L., Stricker, M., Nass Kovács, G., Kloos, M., Doak, B., Shoeman, R. L., et al. (2020). Illumination guidelines for ultrafast pump–probe experiments by serial femtosecond crystallography. Nature methods, 17(7), 681-684. doi:10.1038/s41592-020-0847-3.

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 Creators:
Grünbein, Marie Luise1, Author              
Stricker, Miriam1, Author              
Nass Kovács, Gabriela1, Author              
Kloos, Marco1, Author              
Doak, Bruce1, Author              
Shoeman, Robert L.1, Author              
Reinstein, Jochen1, Author              
Lecler , Sylvain, Author
Haacke , Stefan, Author
Schlichting, Ilme1, Author              
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1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

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 Abstract: Time-resolved crystallography with X-ray free-electron lasers enables structural characterization of light-induced reactions on ultrafast timescales. To be biologically and chemically relevant, such studies must be carried out in an appropriate photoexcitation regime to avoid multiphoton artifacts, a common issue in recent studies. We describe numerical and experimental approaches to determine how many photons are needed for single-photon excitation in microcrystals, taking into account losses by scattering.

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Language(s): eng - English
 Dates: 2019-08-062020-04-172020-05-252020-07-01
 Publication Status: Published in print
 Pages: 9
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 Table of Contents: -
 Rev. Type: Peer
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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 17 (7) Sequence Number: - Start / End Page: 681 - 684 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556