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  Illumination guidelines for ultrafast pump–probe experiments by serial femtosecond crystallography

Grünbein, M. L., Stricker, M., Nass Kovács, G., Kloos, M., Doak, B., Shoeman, R. L., et al. (2020). Illumination guidelines for ultrafast pump–probe experiments by serial femtosecond crystallography. Nature methods, e-pup(47-3), 1-9. doi:10.1038/s41592-020-0847-3.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-7007-4 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-7008-3
Genre: Journal Article

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 Creators:
Grünbein, Marie Luise1, 2, Author              
Stricker, Miriam1, Author              
Nass Kovács, Gabriela1, 3, Author              
Kloos, Marco1, Author              
Doak, Bruce1, 3, Author              
Shoeman, Robert L.1, 3, 4, Author              
Reinstein, Jochen1, 5, 6, Author              
Lecler , Sylvain, Author
Haacke , Stefan, Author
Schlichting, Ilme1, 3, 5, 7, 8, 9, Author              
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
2Max Planck Research Group Behavioural Neurophysiology (Andreas T. Schaefer), Max Planck Institute for Medical Research, Max Planck Society, ou_1497722              
3Coherent diffractive imaging, Max Planck Institute for Medical Research, Max Planck Society, ou_1497692              
4Analytical Protein Biochemistry, Max Planck Institute for Medical Research, Max Planck Society, ou_1497690              
5Abt. III: Physikalische Biochemie, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753289              
6Molecular chaperones, Max Planck Institute for Medical Research, Max Planck Society, ou_1497728              
7Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
8Heme and Flavin Enzymes, Max Planck Institute for Medical Research, Max Planck Society, ou_1497715              
9Photoreceptors, Max Planck Institute for Medical Research, Max Planck Society, ou_1856341              

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 Abstract: Time-resolved crystallography with X-ray free-electron lasers enables structural characterization of light-induced reactions on ultrafast timescales. To be biologically and chemically relevant, such studies must be carried out in an appropriate photoexcitation regime to avoid multiphoton artifacts, a common issue in recent studies. We describe numerical and experimental approaches to determine how many photons are needed for single-photon excitation in microcrystals, taking into account losses by scattering.

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Language(s): eng - English
 Dates: 2019-08-062020-04-172020-05-252020-05-25
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41592-020-0847-3
 Degree: -

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Title: Nature methods
  Other : Nature methods
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: e-pup (47-3) Sequence Number: - Start / End Page: 1 - 9 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556