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  Characteristic views of Escherichia coli RNA polymerase core enzyme in the scanning transmission electron microscope

Tischelaar, W., & Heel, M. v. (1990). Characteristic views of Escherichia coli RNA polymerase core enzyme in the scanning transmission electron microscope. Journal of Structural Biology, 103(2), 180-184. doi:10.1016/1047-8477(90)90021-4.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0006-75E8-1 Version Permalink: https://hdl.handle.net/21.11116/0000-0006-75E9-0
Genre: Journal Article

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 Creators:
Tischelaar, Willem1, Author
Heel, Marin van2, Author           
Affiliations:
1European Molecular Biology Laboratory, Postfach 102209, D-6900 Heidelberg, Germany, ou_persistent22              
2Fritz Haber Institute, Max Planck Society, ou_24021              

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 Abstract: Eight characteristic views of Escherichia coli RNA polymerase core enzyme are presented which were found using multivariate statistical image processing of about 1000 individual molecular images. The data set was obtained from negatively stained specimens with a scanning transmission electron microscope (STEM) operated in dark field mode. The molecular projections were found to a resolution of around 30 Å, thus visualizing new structural details of the core enzyme. An approximate twofold axis seems to relate the β to the β′ subunit as well as both α units to each other.

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Language(s): eng - English
 Dates: 1990-04-241990-03-071990-03-071990-04
 Publication Status: Issued
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/1047-8477(90)90021-4
 Degree: -

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Title: Journal of Structural Biology
  Abbreviation : J. Struct. Biol.
Source Genre: Journal
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Publ. Info: San Diego, CA : Elsevier
Pages: 5 Volume / Issue: 103 (2) Sequence Number: - Start / End Page: 180 - 184 Identifier: ISSN: 1047-8477
CoNE: https://pure.mpg.de/cone/journals/resource/954922650160