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要旨:
Eight characteristic views of Escherichia coli RNA polymerase core enzyme are presented which were found using multivariate statistical image processing of about 1000 individual molecular images. The data set was obtained from negatively stained specimens with a scanning transmission electron microscope (STEM) operated in dark field mode. The molecular projections were found to a resolution of around 30 Å, thus visualizing new structural details of the core enzyme. An approximate twofold axis seems to relate the β to the β′ subunit as well as both α units to each other.
