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  A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata

Zhu, F., Luo, T., Liu, C., Wang, Y., Zheng, L., Xiao, X., et al. (2020). A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata. Journal of Experimental Botany, 71(12), 3613-3625. doi:10.1093/jxb/eraa118.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-9DCE-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-9DCF-1
Genre: Journal Article

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Zhu, Feng1, Author
Luo, Tao1, Author
Liu, Chaoyang1, Author
Wang, Yang1, Author
Zheng, Li1, Author
Xiao, Xue1, Author
Zhang, Mingfei1, Author
Yang, Hongbin1, Author
Yang, Wei1, Author
Xu, Rangwei1, Author
Zeng, Yunliu1, Author
Ye, Junli1, Author
Xu, Juan1, Author
Xu, Jianguo1, Author
Larkin, Robert M1, Author
Wang, Pengwei1, Author
Wen, Weiwei1, Author
Deng, Xiuxin1, Author
Fernie, A. R.2, Author              
Cheng, Yunjiang1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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 Abstract: Although abscisic acid (ABA) is a vital regulator of fruit ripening and several transcription factors have been reported to regulate ABA biosynthesis, reports of the effect of ABA on citrus ripening and the regulation of its biosynthesis by a multiple-transcription-factor complex are scarce. In the present study, a systematic metabolic, cytological, and transcriptome analysis of an ABA-deficient mutant (MT) of Citrus reticulata cv. Suavissima confirmed the positive effect of ABA on the citrus ripening process. The analysis of transcriptome profiles indicated that CrNAC036 played an important role in the ABA deficiency of the mutant, most likely due to an effect on the expression of 9-cis-epoxycarotenoid dioxygenase 5 (CrNCED5). Electrophoretic mobility shift assays and dual luciferase assays demonstrated that CrNAC036 can directly bind and negatively regulate CrNCED5 expression. Furthermore, yeast two-hybrid, bimolecular fluorescence complementation, and dual luciferase assays demonstrated that CrNAC036 interacted with CrMYB68, also down-regulating the expression of CrNCED5. Taken together, our results suggest that CrNAC036 and CrMYB68 synergistically inhibit ABA biosynthesis in citrus fruit by regulating the expression of CrNCED5.

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Language(s): eng - English
 Dates: 2020-062020-06
 Publication Status: Published in print
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 Identifiers: DOI: 10.1093/jxb/eraa118
BibTex Citekey: 10.1093/jxb/eraa118
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Title: Journal of Experimental Botany
  Other : J. Exp. Bot
Source Genre: Journal
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Publ. Info: Oxford : Oxford University Press [etc.]
Pages: - Volume / Issue: 71 (12) Sequence Number: - Start / End Page: 3613 - 3625 Identifier: ISSN: 0022-0957
CoNE: https://pure.mpg.de/cone/journals/resource/954925413883