English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Coding Exon-Structure Aware Realigner (CESAR): Utilizing Genome Alignments for Comparative Gene Annotation.

Sharma, V., & Hiller, M. (2019). Coding Exon-Structure Aware Realigner (CESAR): Utilizing Genome Alignments for Comparative Gene Annotation. Methods in molecular biology (Clifton, N.J.), 1962, 179-191. doi:10.1007/978-1-4939-9173-0_10.

Item is

Files

show Files

Locators

show
hide
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Sharma, Virag1, Author           
Hiller, Michael1, Author           
Affiliations:
1Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              

Content

show
hide
Free keywords: -
 Abstract: Alignment-based gene identification methods utilize sequence conservation between orthologous protein-coding genes to annotate genes in newly sequenced genomes. CESAR is an approach that makes use of existing genome alignments to transfer genes from one genome to other aligned genomes, and thus generates comparative gene annotations. To accurately detect conserved exons that exhibit an intact reading frame and consensus splice sites, CESAR produces a new alignment between orthologous exons, taking information about the exon's reading frame and splice site positions into account. Furthermore, CESAR is able to detect most evolutionary splice site shifts, which helps to annotate exon boundaries at high precision. Here, we describe how to apply CESAR to generate comparative gene annotations for one or many species, and discuss the strengths and limitations of this approach. CESAR is available at https://github.com/hillerlab/CESAR2.0 .

Details

show
hide
Language(s):
 Dates: 2019-01-01
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-4939-9173-0_10
Other: cbg-7255
PMID: 31020560
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Methods in molecular biology (Clifton, N.J.)
  Other : Methods Mol Biol
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 1962 Sequence Number: - Start / End Page: 179 - 191 Identifier: -