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  Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates.

Alberti, S., Gladfelter, A., & Mittag, T. (2019). Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates. Cell, 176(3), 419-434. doi:10.1016/j.cell.2018.12.035.

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 Creators:
Alberti, Simon1, Author           
Gladfelter, Amy, Author
Mittag, Tanja, Author
Affiliations:
1Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              

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 Abstract: Evidence is now mounting that liquid-liquid phase separation (LLPS) underlies the formation of membraneless compartments in cells. This realization has motivated major efforts to delineate the function of such biomolecular condensates in normal cells and their roles in contexts ranging from development to age-related disease. There is great interest in understanding the underlying biophysical principles and the specific properties of biological condensates with the goal of bringing insights into a wide range of biological processes and systems. The explosion of physiological and pathological contexts involving LLPS requires clear standards for their study. Here, we propose guidelines for rigorous experimental characterization of LLPS processes in vitro and in cells, discuss the caveats of common experimental approaches, and point out experimental and theoretical gaps in the field.

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 Dates: 2019-01-24
 Publication Status: Issued
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 Identifiers: DOI: 10.1016/j.cell.2018.12.035
Other: cbg-7315
PMID: 30682370
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Title: Cell
  Other : Cell
Source Genre: Journal
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Pages: - Volume / Issue: 176 (3) Sequence Number: - Start / End Page: 419 - 434 Identifier: -