English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Glycan-dependent cell adhesion mechanism of Tc toxins

Roderer, D., Bröcker, F., Sitsel, O., Kaplonek, P., Leidreiter, F., Seeberger, P. H., et al. (2020). Glycan-dependent cell adhesion mechanism of Tc toxins. Nature Communications, 11: 2694, pp. 1-13. doi:10.1038/s41467-020-16536-7.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0006-808B-C Version Permalink: http://hdl.handle.net/21.11116/0000-0006-808C-B
Genre: Journal Article

Files

show Files
hide Files
:
NatCommun_11_2020_2694.pdf (Any fulltext), 4MB
 
File Permalink:
-
Name:
NatCommun_11_2020_2694.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
NatCommun_11_2020_2694_Movie1.mp4 (Supplementary material), 34MB
 
File Permalink:
-
Name:
NatCommun_11_2020_2694_Movie1.mp4
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/mp4
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
NatCommun_11_2020_2694_Movie2.mp4 (Supplementary material), 35MB
 
File Permalink:
-
Name:
NatCommun_11_2020_2694_Movie2.mp4
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/mp4
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
NatCommun_11_2020_2694_Movie3.mp4 (Supplementary material), 31MB
 
File Permalink:
-
Name:
NatCommun_11_2020_2694_Movie3.mp4
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/mp4
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
NatCommun_11_2020_2694_Suppl1.pdf (Supplementary material), 20MB
 
File Permalink:
-
Name:
NatCommun_11_2020_2694_Suppl1.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
NatCommun_11_2020_2694_Suppl2.pdf (Supplementary material), 241KB
 
File Permalink:
-
Name:
NatCommun_11_2020_2694_Suppl2.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
NatCommun_11_2020_2694_Suppl3.pdf (Supplementary material), 85KB
 
File Permalink:
-
Name:
NatCommun_11_2020_2694_Suppl3.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
NatCommun_11_2020_2694_Suppl4.pdf (Supplementary material), 158KB
 
File Permalink:
-
Name:
NatCommun_11_2020_2694_Suppl4.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
NatCommun_11_2020_2694_Suppl5.xlsx (Supplementary material), 714KB
 
File Permalink:
-
Name:
NatCommun_11_2020_2694_Suppl5.xlsx
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Roderer, Daniel, Author              
Bröcker, Felix, Author
Sitsel, Oleg, Author              
Kaplonek, Paulina, Author
Leidreiter, Franziska1, Author              
Seeberger, Peter H., Author
Raunser, Stefan, Author              
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Content

show
hide
Free keywords: -
 Abstract: Toxin complex (Tc) toxins are virulence factors of pathogenic bacteria. Tcs are composed of three subunits: TcA, TcB and TcC. TcA facilitates receptor–toxin interaction and membrane permeation, TcB and TcC form a toxin-encapsulating cocoon. While the mechanisms of holotoxin assembly and pore formation have been described, little is known about receptor binding of TcAs. Here, we identify heparins/heparan sulfates and Lewis antigens as receptors for different TcAs from insect and human pathogens. Glycan array screening reveals that all tested TcAs bind negatively charged heparins. Cryo-EM structures of Morganella morganii TcdA4 and Xenorhabdus nematophila XptA1 reveal that heparins/heparan sulfates unexpectedly bind to different regions of the shell domain, including receptor-binding domains. In addition, Photorhabdus luminescens TcdA1 binds to Lewis antigens with micromolar affinity. Here, the glycan interacts with the receptor-binding domain D of the toxin. Our results suggest a glycan dependent association mechanism of Tc toxins on the host cell surface.

Details

show
hide
Language(s): eng - English
 Dates: 2020-06-01
 Publication Status: Published online
 Pages: 13
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/s41467-020-16536-7
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 11 Sequence Number: 2694 Start / End Page: 1 - 13 Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723