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  Temporal expression of MOF acetyltransferase primes transcription factor networks for erythroid fate

Rodrigues, C. P., Herman, J. S., Herquel, B., Velsecchi Keller, C. I., Stehle, T., Grün, D., et al. (2020). Temporal expression of MOF acetyltransferase primes transcription factor networks for erythroid fate. Science Advances, 6, eaaz4815. doi:10.1126/sciadv.aaz4815.

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eaaz4815.full.pdf (Publisher version), 4MB
 
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Rodrigues et al.
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Restricted (Max Planck Institute of Immunobiology and Epigenetics, MFIB; )
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application/pdf
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Copyright Date:
2020
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cc-by-nc/4.0

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 Creators:
Rodrigues, Cecilia Pessoa1, Author
Herman, Josip Stefan1, Author
Herquel, Benjamin1, Author
Velsecchi Keller, Claudia Isabelle1, Author
Stehle, Thomas1, Author              
Grün, Dominic1, Author              
Akhtar, Asifa1, Author              
Affiliations:
1Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243640              

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 Abstract: Self-renewal and differentiation of hematopoietic stem cells (HSCs) are orchestrated by the combinatorial action of transcription factors and epigenetic regulators. Here, we have explored the mechanism by which histone H4 lysine 16 acetyltransferase MOF regulates erythropoiesis. Single-cell RNA sequencing and chromatin immunoprecipitation sequencing uncovered that MOF influences erythroid trajectory by dynamic recruitment to chromatin and its haploinsufficiency causes accumulation of a transient HSC population. A regulatory network consisting of MOF, RUNX1, and GFI1B is critical for erythroid fate commitment. GFI1B acts as a Mof activator which is necessary and sufficient for cell type-specific induction of Mof expression. Plasticity of Mof-depleted HSCs can be rescued by expression of a downstream effector, Gata1, or by rebalancing acetylation via a histone deacetylase inhibitor. Accurate timing and dosage of Mof expression act as a rheostat for the feedforward transcription factor network that safeguards progression along the erythroid fate.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/sciadv.aaz4815
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Title: Science Advances
  Other : Sci. Adv.
Source Genre: Journal
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Publ. Info: Washington : AAAS
Pages: - Volume / Issue: 6 Sequence Number: - Start / End Page: eaaz4815 Identifier: ISSN: 2375-2548
CoNE: https://pure.mpg.de/cone/journals/resource/2375-2548