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  Quantifying molecule numbers in STED/RESOLFT fluorescence nanoscopy

Keller-Findeisen, J., Sahl, S. J., & Hell, S. W. (2020). Quantifying molecule numbers in STED/RESOLFT fluorescence nanoscopy. In Nanoscale Photonic Imaging (pp. 205-226). Berlin, Göttingen, Heidelberg: Springer, Cham.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-8B4D-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-8B5B-8
Genre: Book Chapter

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NanoPhotonicImaging_Chap7_2020_205.pdf (Any fulltext), 728KB
 
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 Creators:
Keller-Findeisen, Jan, Author
Sahl, Steffen J., Author
Hell, Stefan W.1, Author              
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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Free keywords: Higher order effects, Molecular contribution function, Molecular counting, Nanoscopy, Photon antibunching, Photoswitching, PSF, RESOLFT, Resolution, Saturation, STED
 Abstract: Quantification of the numbers of molecules of interest in the specimen has emerged as a powerful capability of several fluorescence nanoscopy approaches. Carefully relating the measured signals from STED or RESOLFT scanning nanoscopy data to the contribution of a single molecule, reliable estimates of fluorescent molecule numbers can be obtained. To achieve this, higher-order signatures in the obtained photon statistics are analyzed, as arise from the antibunched nature of single-fluorophore emissions or in the signal variance among multiple on/off-switching cycles. In this chapter, we discuss the concepts and approaches demonstrated to date for counting molecules in STED/RESOLFT nanoscopy.

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Language(s): eng - English
 Dates: 2020-06-10
 Publication Status: Published in print
 Pages: 22
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-3-030-34413-9_7
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Title: Nanoscale Photonic Imaging
Source Genre: Book
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Publ. Info: Berlin, Göttingen, Heidelberg : Springer, Cham
Pages: - Volume / Issue: Chapter 7 Sequence Number: - Start / End Page: 205 - 226 Identifier: ISBN: 978-3-030-34412-2
ISBN: 978-3-030-34413-9