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  An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases

Bittmann, J., Grigaitis, R., Galanti, L., Amarell, S., Wilfling, F., Matos, J., et al. (2020). An advanced cell cycle tag toolbox reveals principles underlying temporal control of structure-selective nucleases. ELIFE, 9: e52459. doi:10.7554/eLife.52459.

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 Creators:
Bittmann, Julia1, Author              
Grigaitis, Rokas2, Author
Galanti, Lorenzo1, Author              
Amarell, Silas1, Author              
Wilfling, Florian3, Author              
Matos, Joao2, Author
Pfander, Boris1, Author              
Affiliations:
1Pfander, Boris / DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565165              
2external, ou_persistent22              
3Jentsch, Stefan / Molecular Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565156              

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Free keywords: HOLLIDAY JUNCTION RESOLUTION; DNA-DAMAGE TOLERANCE; SACCHAROMYCES-CEREVISIAE MUS81-MMS4; STRUCTURE-SPECIFIC ENDONUCLEASES; BLOOMS-SYNDROME HELICASE; REPLICATION FORK; S-PHASE; SUBSTRATE-SPECIFICITY; FUNCTIONAL OVERLAP; COMPLEX PROMOTES
 Abstract: Cell cycle tags allow to restrict target protein expression to specific cell cycle phases. Here, we present an advanced toolbox of cell cycle tag constructs in budding yeast with defined and compatible peak expression that allow comparison of protein functionality at different cell cycle phases. We apply this technology to the question of how and when Mus81-Mms4 and Yen1 nucleases act on DNA replication or recombination structures. Restriction of Mus81-Mms4 to M phase but not S phase allows a wildtype response to various forms of replication perturbation and DNA damage in S phase, suggesting it acts as a post-replicative resolvase. Moreover, we use cell cycle tags to reinstall cell cycle control to a deregulated version of Yen1, showing that its premature activation interferes with the response to perturbed replication. Curbing resolvase activity and establishing a hierarchy of resolution mechanisms are therefore the principal reasons underlying resolvase cell cycle regulation.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published online
 Pages: 29
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000535191000001
DOI: 10.7554/eLife.52459
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Title: ELIFE
Source Genre: Journal
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Publ. Info: SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND : ELIFE SCIENCES PUBLICATIONS LTD
Pages: - Volume / Issue: 9 Sequence Number: e52459 Start / End Page: - Identifier: ISSN: 2050-084X