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  Ligand diffusion enables force‐independent cell adhesion via activating α5β1 integrin and initiating rac and RhoA signaling

Yu, L., Hou, Y., Xie, W., Camacho, J. L. C., Cheng, C., Holle, A. W., et al. (2020). Ligand diffusion enables force‐independent cell adhesion via activating α5β1 integrin and initiating rac and RhoA signaling. Advanced Materials, e-pup(2002566), 1-12. doi:10.1002/adma.202002566.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-9533-8 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-9534-7
Genre: Journal Article

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 Creators:
Yu , Leixiao, Author
Hou, Yong, Author
Xie, Wenyan, Author
Camacho, Jose Luis Cuellar, Author
Cheng, Chong, Author
Holle, Andrew W.1, 2, Author              
Young, Jennifer L.1, 2, Author              
Trappmann, Britta, Author
Zhao, Weifeng, Author
Melzig, Matthias F., Author
Cavalcanti-Adam, Elisabetta Ada1, 2, Author              
Zhao, Changsheng, Author
Spatz, Joachim P.1, 2, Author              
Wei, Qiang, Author
Haag, Rainer, Author
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: adhesive ligands, biointerfaces, cell adhesion, mechanotransduction, polymer coatings
 Abstract: Cells reside in a dynamic microenvironment in which adhesive ligand availability, density, and diffusivity are key factors regulating cellular behavior. Here, the cellular response to integrin‐binding ligand dynamics by directly controlling ligand diffusivity via tunable ligand–surface interactions is investigated. Interestingly, cell spread on the surfaces with fast ligand diffusion is independent of myosin‐based force generation. Fast ligand diffusion enhances α5β1 but not αvβ3 integrin activation and initiates Rac and RhoA but not ROCK signaling, resulting in lamellipodium‐based fast cell spreading. Meanwhile, on surfaces with immobile ligands, αvβ3 and α5β1 integrins synergistically initiate intracellular‐force‐based canonical mechanotransduction pathways to enhance cell adhesion and osteogenic differentiation of stem cells. These results indicate the presence of heretofore‐unrecognized pathways, distinct from canonical actomyosin‐driven mechanisms, that are capable of promoting cell adhesion.

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Language(s): eng - English
 Dates: 2020-04-152020-05-122020-06-14
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1002/adma.202002566
 Degree: -

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Title: Advanced Materials
  Other : Adv. Mater.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: e-pup (2002566) Sequence Number: - Start / End Page: 1 - 12 Identifier: ISSN: 0935-9648
CoNE: https://pure.mpg.de/cone/journals/resource/954925570855