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  A method for preparing a functional synthetic cell in form of a giant unilamellar vesicle
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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-9572-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-9573-0
Genre: Patent

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US_20200170949.pdf (Any fulltext), 727KB
 
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 Creators:
Spatz, Joachim P.1, 2, Author              
Benk, Lucia T., Author
Frohnmayer, Johannes Patrick, Author
Haller, Barbara, Author
Janiesch, Jan-willi, Author
Platzman, Ilia1, 2, Author              
Weiss, Marian1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Physical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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 Abstract: The present invention relates to a method for preparing a protocell in form of a giant unilamellar vesicle, which comprises the following steps: a) providing a water-based droplet encapsulated by an outer polymer shell, which borders the inner space of the droplet, wherein the droplet has a maximum dimension of 0.5 μm to 1,000 μm, wherein the inner space of the droplet contains at least one lipid, b) transforming the lipid content of the droplet into a lipid bilayer which is arranged at and covers the inner surface of the polymer shell and oil phase in order to form a polymer shell-stabilized giant unilamellar vesicle, c) optionally incorporating one or more proteins and/or nuclei into the polymer shell-stabilized giant unilamellar vesicle provided in step b) and d) optionally removing the polymer shell and oil phase from the polymer shell-stabilized giant unilamellar vesicle and optionally transferring it from the oil to the water phase.

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 Dates: 2019-06-062020-04-06
 Publication Status: Published in print
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