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  Multimodal fast optical interrogation of neural circuitry

Zhang, F., Wang, L.-P., Brauner, M., Liewald, J. F., Kay, K., Watzke, N., et al. (2007). Multimodal fast optical interrogation of neural circuitry. Nature, 446(7136), 633-639. doi:10.1038/nature05744.

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 Creators:
Zhang, Feng1, Author
Wang, Li-Ping1, Author
Brauner, Martin2, Author
Liewald, Jana F.2, Author
Kay, Kenneth1, Author
Watzke, Natalie3, Author           
Wood, Phillip G.3, Author           
Bamberg, Ernst3, 4, Author           
Nagel, Georg3, 5, Author           
Gottschalk, Alexander2, Author
Deisseroth, Karl1, Author
Affiliations:
1Department of Bioengineering, Stanford University, Stanford, California, USA, ou_persistent22              
2Institute of Biochemistry, Department of Biochemistry, Chemistry and Pharmacy, Johann Wolfgang Goethe-University, Frankfurt, Germany, ou_persistent22              
3Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
4Institute of Biophysical Chemistry, Department of Biochemistry, Chemistry and Pharmacy, Johann Wolfgang Goethe-University, Frankfurt, Germany, ou_persistent22              
5University Würzburg, Botanik I, Würzburg, Germany, ou_persistent22              

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Free keywords: Action Potentials, Animals, Animals, Genetically Modified, Brain, Caenorhabditis elegans, Calcium, Chlorides, Electrophysiology, Halorhodopsins, Hippocampus, Light, Mice, Nerve Net, Neural Pathways, Neurons, Oocytes, Optics and Photonics, Rats, Rhodopsin, Time Factors
 Abstract: Our understanding of the cellular implementation of systems-level neural processes like action, thought and emotion has been limited by the availability of tools to interrogate specific classes of neural cells within intact, living brain tissue. Here we identify and develop an archaeal light-driven chloride pump (NpHR) from Natronomonas pharaonis for temporally precise optical inhibition of neural activity. NpHR allows either knockout of single action potentials, or sustained blockade of spiking. NpHR is compatible with ChR2, the previous optical excitation technology we have described, in that the two opposing probes operate at similar light powers but with well-separated action spectra. NpHR, like ChR2, functions in mammals without exogenous cofactors, and the two probes can be integrated with calcium imaging in mammalian brain tissue for bidirectional optical modulation and readout of neural activity. Likewise, NpHR and ChR2 can be targeted together to Caenorhabditis elegans muscle and cholinergic motor neurons to control locomotion bidirectionally. NpHR and ChR2 form a complete system for multimodal, high-speed, genetically targeted, all-optical interrogation of living neural circuits.

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Language(s): eng - English
 Dates: 2006-12-232007-03-142007-04-05
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nature05744
BibTex Citekey: zhang_multimodal_2007
 Degree: -

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Title: Nature
  Abbreviation : Nature
Source Genre: Journal
 Creator(s):
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 446 (7136) Sequence Number: - Start / End Page: 633 - 639 Identifier: ISSN: 0028-0836
CoNE: https://pure.mpg.de/cone/journals/resource/954925427238