English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Microfluidic trapping of vesicles reveals membrane-tension dependent FtsZ cytoskeletal re-organisation

Ganzinger, K. A., Merino-Salomon, A., Garcia-Soriano, D., Butterfield, N., Butterfield, N. A., Litschel, T., et al. (2019). Microfluidic trapping of vesicles reveals membrane-tension dependent FtsZ cytoskeletal re-organisation. bioRxiv, 791459. doi:10.1101/791459.

Item is

Files

show Files
hide Files
:
791459v1.full.pdf (Preprint), 3MB
Name:
791459v1.full.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.

Locators

show

Creators

show
hide
 Creators:
Ganzinger, Kristina A.1, Author           
Merino-Salomon, Adrian1, Author           
Garcia-Soriano, Daniela1, Author           
Butterfield, Nelson1, Author           
Butterfield, Nelson A.1, Author           
Litschel, Thomas1, Author           
Siedler, Frank1, Author           
Schwille, Petra1, Author           
Affiliations:
1Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

Content

show
hide
Free keywords: -
 Abstract: The geometry of reaction compartments can affect the outcome of chemical reactions. Synthetic biology commonly uses giant unilamellar vesicles (GUVs) to generate cell-sized, membrane-bound reaction compartments. However, these liposomes are always spherical due to surface area minimization. Here, we have developed a microfluidic chip to trap and reversibly deform GUVs into rod- or cigar-like shapes, including a constriction site in the trap mimicking the membrane furrow in cell division. When we introduce into these GUVs the bacterial tubulin homologue FtsZ, the primary protein of the bacterial Z ring, we find that FtsZ organization changes from dynamic rings to elongated filaments upon GUV deformation, and that these FtsZ filaments align preferentially with the short GUV axis, in particular at the membrane neck. In contrast, pulsing Min oscillations in GUVs remained largely unaffected. We conclude that microfluidic traps are a useful tool for deforming GUVs into non-spherical membrane shapes, akin to those seen in cell division, and for investigating the effect of confinement geometry on biochemical reactions, such as protein filament self-organization.

Details

show
hide
Language(s):
 Dates: 2019
 Publication Status: Published online
 Pages: 25
 Publishing info: bioRxiv
 Table of Contents: -
 Rev. Type: No review
 Identifiers: DOI: 10.1101/791459
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: bioRxiv
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: 791459 Start / End Page: - Identifier: -