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Abstract:
In this chapter we will give first an overview about the many functions of the cytoskeleton, its composition and structure in various organisms. We will describe next methods to anchor cytoskeletal filaments to membranes of supported lipid bilayers (SLB) and giant unilamellar vesicles (GUV) by several strategies based on charge, amphipathic helices, streptavidin-biotin binding or by integral membrane proteins. Will follow a description of recent bottom-up applications referring to all kingdoms of live, e.g., mimicries of actin cortices of eukaryotic cells, FtsZ-FtsA filaments of Escherichia coli, and CdvABC membrane deformation as in the archaea Sulfolobus acidocaldarius. The chapter closes with a summary and an outlook into the future of this interesting research direction.
