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  Point spread function in interferometric scattering microscopy (iSCAT). Part I: aberrations in defocusing and axial localization

Gholami Mahmoodabadi, R., Taylor, R. W., Kaller, M., Spindler, S., Mazaheri, M., Kasaian, K., et al. (2020). Point spread function in interferometric scattering microscopy (iSCAT). Part I: aberrations in defocusing and axial localization. Optics Express, 28, 25969-25988. doi:10.1364/OE.401374.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-9F89-D Version Permalink: http://hdl.handle.net/21.11116/0000-0006-EBBC-E
Genre: Journal Article

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 Creators:
Gholami Mahmoodabadi, Reza1, 2, Author              
Taylor, Richard W.1, 2, Author              
Kaller, Martin1, 2, Author              
Spindler, Susann1, Author              
Mazaheri, Mahdi1, Author
Kasaian, Kiarash1, Author
Sandoghdar, Vahid1, 2, 3, Author              
Affiliations:
1Sandoghdar Division, Max Planck Institute for the Science of Light, Max Planck Society, ou_2364722              
2Max-Planck-Zentrum für Physik und Medizin, Max Planck Institute for the Science of Light, Max Planck Society, ou_3164414              
3Department of Physics, Friedrich Alexander University, Erlangen, Germany, ou_persistent22              

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 Abstract: Interferometric scattering (iSCAT) microscopy is an emerging label-free technique optimized for the sensitive detection of nano-matter. Previous iSCAT studies have approximated the point spread function in iSCAT by a Gaussian intensity distribution. However, recent efforts to track the mobility of nanoparticles in challenging speckle environments and over extended axial ranges has necessitated a quantitative description of the interferometric point spread function (iPSF). We present a robust vectorial diffraction model for the iPSF in tandem with experimental measurements and rigorous FDTD simulations. We examine the iPSF under various imaging scenarios to understand how aberrations due to the experimental configuration encode information about the nanoparticle. We show that the lateral shape of the iPSF can be used to achieve nanometric three-dimensional localization over an extended axial range on the order of 10 µm either by means of a fit to an analytical model or calibration-free unsupervised machine learning. Our results have immediate implications for three-dimensional single particle tracking in complex scattering media.

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Language(s): eng - English
 Dates: 2020-06-272020-08-31
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1364/OE.401374
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Title: Optics Express
  Abbreviation : Opt. Express
Source Genre: Journal
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Publ. Info: Washington, DC : Optical Society of America
Pages: - Volume / Issue: 28 Sequence Number: - Start / End Page: 25969 - 25988 Identifier: ISSN: 1094-4087
CoNE: https://pure.mpg.de/cone/journals/resource/954925609918