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  The bicistronic gene würmchen encodes two essential components for epithelial development in Drosophila

Königsmann, T., Parfentev, I., Urlaub, H., Riedel, D., & Schuh, R. (2020). The bicistronic gene würmchen encodes two essential components for epithelial development in Drosophila. Developmental Biology, 463(1), 53-62. doi:10.1016/j.ydbio.2020.04.005.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-A0E6-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-A0F1-4
Genre: Journal Article

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 Creators:
Königsmann, T.1, Author              
Parfentev, I.2, Author              
Urlaub, H.2, Author              
Riedel, D.3, Author              
Schuh, R.1, Author              
Affiliations:
1Research Group of Molecular Organogenesis, MPI for Biophysical Chemistry, Max Planck Society, ou_578591              
2Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              
3Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              

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Free keywords: Septate junction; Drosophila; Bicistronic; Barrier function; Core complex
 Abstract: Epithelial tissues are fundamental for the establishment and maintenance of different body compartments in multicellular animals. To achieve this specific task epithelial sheets secrete an apical extracellular matrix for tissue strength and protection and they organize a transepithelial barrier function, which is mediated by tight junctions in vertebrates or septate junctions in invertebrates. Here, we show that the bicistronic gene würmchen is functionally expressed in epithelial tissues. CRISPR/Cas9-mediated mutations in both coding sequences reveal two essential polypeptides, Würmchen1 and Würmchen2, which are both necessary for normal epithelial tissue development. Würmchen1 represents a genuine septate junction core component. It is required during embryogenesis for septate junction organization, the establishment of a transepithelial barrier function, distinct cellular transport processes and tracheal system morphogenesis. Würmchen2 is localized in the apical membrane region of epithelial tissues and in a central core of the tracheal lumen during embryogenesis. It is essential during the later larval development.

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Language(s): eng - English
 Dates: 2020-05-012020-07-01
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.ydbio.2020.04.005
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Title: Developmental Biology
Source Genre: Journal
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Pages: - Volume / Issue: 463 (1) Sequence Number: - Start / End Page: 53 - 62 Identifier: -