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  Structural Insights Into Transcription Initiation by Yeast RNA Polymerase I

Sadian, Y., Tafur, L., Kosinski, J., Jakobi, A. J., Wetzel, R., Buczak, K., et al. (2017). Structural Insights Into Transcription Initiation by Yeast RNA Polymerase I. The EMBO Journal, 36(18), 2698-2709. doi:10.15252/embj.201796958.

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 Creators:
Sadian, Yashar1, Author
Tafur, Lucas1, Author
Kosinski, Jan1, Author
Jakobi, Arjen J.1, Author
Wetzel, Rene1, Author
Buczak, Katarzyna1, Author
Hagen, Wim Jh1, Author
Beck, Martin2, Author              
Sachse, Carsten1, Author
Müller, Christoph W.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2European Molecular Biology Laboratory (EMBL), Heidelberg, Germany, ou_persistent22              

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Free keywords: Core Factor, Cryoelectron Microscopy, DNA, Fungal, electron cryo‐microscopy, Models, Molecular, Pol I pre‐initiation complex, Protein Conformation, Protein Multimerization, ribosomal RNA, RNA Polymerase I, RNA, Ribosomal, Saccharomyces cerevisiae, transcription, Transcription, Genetic
 Abstract: In eukaryotic cells, RNA polymerase I (Pol I) synthesizes precursor ribosomal RNA (pre-rRNA) that is subsequently processed into mature rRNA. To initiate transcription, Pol I requires the assembly of a multi-subunit pre-initiation complex (PIC) at the ribosomal RNA promoter. In yeast, the minimal PIC includes Pol I, the transcription factor Rrn3, and Core Factor (CF) composed of subunits Rrn6, Rrn7, and Rrn11. Here, we present the cryo-EM structure of the 18-subunit yeast Pol I PIC bound to a transcription scaffold. The cryo-EM map reveals an unexpected arrangement of the DNA and CF subunits relative to Pol I. The upstream DNA is positioned differently than in any previous structures of the Pol II PIC. Furthermore, the TFIIB-related subunit Rrn7 also occupies a different location compared to the Pol II PIC although it uses similar interfaces as TFIIB to contact DNA. Our results show that although general features of eukaryotic transcription initiation are conserved, Pol I and Pol II use them differently in their respective transcription initiation complexes.

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Language(s): eng - English
 Dates: 2017-06-202017-03-172017-06-262017-07-242017-09-15
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.15252/embj.201796958
BibTex Citekey: sadian_structural_2017
 Degree: -

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Title: The EMBO Journal
Source Genre: Journal
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Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 36 (18) Sequence Number: - Start / End Page: 2698 - 2709 Identifier: ISSN: 0261-4189
CoNE: https://pure.mpg.de/cone/journals/resource/954925497061_1