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  The Combination of X-Ray Crystallography and Cryo-Electron Microscopy Provides Insight Into the Overall Architecture of the Dodecameric Rvb1/Rvb2 Complex

Silva-Martin, N., Daudén, M. I., Glatt, S., Hoffmann, N. A., Kastritis, P., Bork, P., et al. (2016). The Combination of X-Ray Crystallography and Cryo-Electron Microscopy Provides Insight Into the Overall Architecture of the Dodecameric Rvb1/Rvb2 Complex. PLoS One, 11(1): e0146457. doi:10.1371/journal.pone.0146457.

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 Creators:
Silva-Martin, Noella1, Author
Daudén, María I.1, Author
Glatt, Sebastian1, Author
Hoffmann, Niklas A.1, Author
Kastritis, Panagiotis1, Author
Bork, Peer1, Author
Beck, Martin2, Author              
Müller, Christoph W.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2European Molecular Biology Laboratory (EMBL), Heidelberg, Germany, ou_persistent22              

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Free keywords: Catalytic Domain, Chaetomium, Cryoelectron Microscopy, Crystallography, X-Ray, DNA Helicases, DNA-Binding Proteins, Fungal Proteins, Models, Molecular, Protein Interaction Domains and Motifs, Protein Structure, Quaternary, Protein Structure, Secondary
 Abstract: The Rvb1/Rvb2 complex is an essential component of many cellular pathways. The Rvb1/Rvb2 complex forms a dodecameric assembly where six copies of each subunit form two heterohexameric rings. However, due to conformational variability, the way the two rings pack together is still not fully understood. Here, we present the crystal structure and two cryo-electron microscopy reconstructions of the dodecameric, full-length Rvb1/Rvb2 complex, all showing that the interaction between the two heterohexameric rings is mediated through the Rvb1/Rvb2-specific domain II. Two conformations of the Rvb1/Rvb2 dodecamer are present in solution: a stretched conformation also present in the crystal, and a compact conformation. Novel asymmetric features observed in the reconstruction of the compact conformation provide additional insight into the plasticity of the Rvb1/Rvb2 complex.

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Language(s): eng - English
 Dates: 2015-10-062015-12-172016-01-08
 Publication Status: Published online
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1371/journal.pone.0146457
BibTex Citekey: silva-martin_combination_2016
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 11 (1) Sequence Number: e0146457 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850