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  Observation of Mg2+.ATP and uncomplexed ATP in slow exchange by 31P-NMR at high magnetic fields

Sontheimer, G. M., Kuhn, W., & Kalbitzer, H. R. (1986). Observation of Mg2+.ATP and uncomplexed ATP in slow exchange by 31P-NMR at high magnetic fields. Biochemical and Biophysical Research Communications, 134(3), 1379-1386. doi:10.1016/0006-291X(86)90402-X.

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BiochemBiophysResComm_134_1986_1379.pdf (Any fulltext), 298KB
 
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Sontheimer, Gerhard M.1, Author           
Kuhn, W., Author
Kalbitzer, Hans Robert2, Author           
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1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Abstract: The 31P-NMR lines of the β-phosphate groups in Mg2+.ATP and in metalfree ATP can be observed separately up to 280 K at 8.5 T and up to 285 K at 11.7 T. At 274 K and 8.5T the β-phosporous resonances are in slow exchange at pH values above pH 5, the γ-phosporous resonances are in slow exchange only near pH 6, but in fast exchange at low and high pH-values. The fast exchange condition holds for the α-phosphorous resonances over the entire pH-range. For Ca2+.ATP and metalfree ATP always fast exchange prevails down to the freezing point of water even at 11.7 T. Based on the separate observation of the 31P-NMR signals of Mg2+.ATP and uncomplexed ATP new experiments are proposed and possible sources of error in ‘in vivo’ NMR studies are discussed.

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Language(s): eng - English
 Dates: 1985-12-202004-12-081986-02-13
 Publication Status: Issued
 Pages: 8
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/0006-291X(86)90402-X
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Title: Biochemical and Biophysical Research Communications
  Other : Biochem. Biophys. Res. Commun.
Source Genre: Journal
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Publ. Info: Orlando, Fla. : Academic Press
Pages: - Volume / Issue: 134 (3) Sequence Number: - Start / End Page: 1379 - 1386 Identifier: ISSN: 0006-291X
CoNE: https://pure.mpg.de/cone/journals/resource/954922652205_1