English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  α-Amanitin uptake into hepatocytes. Identification of hepatic membrane transport systems used by amatoxins

Kröncke, K. D., Fricker, G., Meier, P. J., Gerok, W., Wieland, T., & Kurz, G. (1986). α-Amanitin uptake into hepatocytes. Identification of hepatic membrane transport systems used by amatoxins. journal of biological chemistry, 261(27), 12562-12567. Retrieved from https://www.jbc.org/content/261/27/12562.abstract.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0006-B3D7-D Version Permalink: http://hdl.handle.net/21.11116/0000-0006-B3D8-C
Genre: Journal Article

Files

show Files
hide Files
:
JBiolChem_261_1986_12562.pdf (Any fulltext), 747KB
 
File Permalink:
-
Name:
JBiolChem_261_1986_12562.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Kröncke, K. D., Author
Fricker, G., Author
Meier, P. J., Author
Gerok, W., Author
Wieland, Theodor1, Author              
Kurz, G., Author
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              

Content

show
hide
Free keywords: -
 Abstract: Hepatic transport studies with amatoxins, toxic bicyclic octapeptides from poisonous mushrooms of the genus Amanita were performed, using [(6'-O,1'-N-di[3H]methyl)trp4]-alpha-amanitin and [(6'-O,1'-N-di-methyl)trp4]-[4-[3H]desmethyl)hyi3]-gamma-ama nitin. Uptake into hepatocytes from rat liver was inhibited by taurocholate and antamanide. Photoaffinity labeling studies with isolated hepatocytes and basolateral plasma membranes, using the sodium salt of (7,7-azo-3 alpha, 12 alpha-dihydroxy-5 beta-[3 beta-3H]cholan-24-oyl)-2- aminoethanesulfonic acid demonstrated that the presence of alpha-amanitin decreased the labeling of the two sinusoidal bile salt-binding membrane polypeptides with the apparent molecular weights of 54,000 and 48,000. In basolateral plasma membrane vesicles amanitin uptake was temperature-dependent and could be stimulated 1.5 to 2-fold by an out to in Na+ gradient as compared to a K+ gradient or sucrose and 2 to 2.5-fold as compared to amanitin equilibration (overshoot). Kinetic studies proved saturability of amanitin uptake in the presence and absence of a Na+ gradient. Membrane transport could be inhibited by taurocholate, antamanide, phalloidin, prednisolone, and silybin, but not by penicillin G or thioctic acid. Hepatic uptake of amatoxins is mediated by the sinusoidal bile salt-transport systems which are also involved in the uptake of antamanide and phalloidin. This supports the concept of a multispecificity of hepatic transport systems for a wide variety of amphipathic molecules.

Details

show
hide
Language(s): eng - English
 Dates: 1985-12-061986-09-25
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: journal of biological chemistry
Source Genre: Encyclopedia
 Creator(s):
Affiliations:
Publ. Info: United States : Baltimore, MD
Pages: - Volume / Issue: 261 (27) Sequence Number: - Start / End Page: 12562 - 12567 Identifier: ISSN: 0021-9258
ISSN: 1083-351X