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  A combinatorial code of transcription factors specifies subtypes of visual motion-sensing neurons in Drosophila

Hörmann, N., Schilling, T., Ali, A. H., Serbe, E., Mayer, C., Borst, A., et al. (2020). A combinatorial code of transcription factors specifies subtypes of visual motion-sensing neurons in Drosophila. Development, 147(9): dev186296. doi:10.1242/dev.186296.

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Data availability: Raw sequencing data (fastq-files) are freely available from NCBI Gene Expression Omnibus under accession number GSE147987. Code is freely available from GitHub (github.com/borstlab/TF_code_paper).
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 Creators:
Hörmann, Nikolai1, Author              
Schilling, Tabea1, Author              
Ali, Aicha Haji1, Author              
Serbe, Etienne1, Author              
Mayer, Christian2, Author              
Borst, Alexander1, Author              
Pujol-Marti, Jesus1, Author              
Affiliations:
1Department: Circuits-Computation-Models / Borst, MPI of Neurobiology, Max Planck Society, ou_1113548              
2Research Group: Neurogenomics / Mayer, MPI of Neurobiology, Max Planck Society, ou_3060203              

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Free keywords: AXON GUIDANCE; OPTIC LOBE; MELANOGASTER; PATHWAYS; CIRCUIT; SPECIFICATION; PRINCIPLES; DETECTORS; SELECTORS; PROTEINSDrosophila; Dendrite development; Motion vision; Neuronal subtypes; Combinatorial code; Grain;
 Abstract: Direction-selective T4/T5 neurons exist in four subtypes, each tuned to visual motion along one of the four cardinal directions. Along with their directional tuning, neurons of each T4/T5 subtype orient their dendrites and project their axons in a subtype-specific manner. Directional tuning, thus, appears strictly linked to morphology in T4/T5 neurons. How the four T4/T5 subtypes acquire their distinct morphologies during development remains largely unknown. Here, we investigated when and how the dendrites of the four T4/T5 subtypes acquire their specific orientations, and profiled the transcriptomes of all T4/T5 neurons during this process. This revealed a simple and stable combinatorial code of transcription factors defining the four T4/T5 subtypes during their development. Changing the combination of transcription factors of specific T4/T5 subtypes resulted in predictable and complete conversions of subtype-specific properties, i.e. dendrite orientation and matching axon projection pattem. Therefore, a combinatorial code of transcription factors coordinates the development of dendrite and axon morphologies to generate anatomical specializations that differentiate subtypes of T4/T5 motion-sensing neurons.

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Language(s): eng - English
 Dates: 2020-05
 Publication Status: Published online
 Pages: 14
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 Table of Contents: We also thank M. Oster (Imaging Facility, MPI of Biochemistry), R. Kim, R. Gautsch, M. Driessen (NGS core facility, MPI of Biochemistry) and A. Yeroslaviz (Cox lab, MPI of Biochemistry) for assistance with scRNA-seq experiments and analysis; M. Sauter for technical assistance with optic lobe dissections; R. Kasper (Imaging Facility, MPI of Neurobiology) for technical assistance with confocal imaging.
 Rev. Type: Peer
 Identifiers: ISI: 000541745300008
DOI: 10.1242/dev.186296
PMID: 32238425
 Degree: -

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Title: Development
  Other : Development
Source Genre: Journal
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Publ. Info: Cambridge, Cambridgeshire : Company of Biologists
Pages: - Volume / Issue: 147 (9) Sequence Number: dev186296 Start / End Page: - Identifier: ISSN: 0950-1991
CoNE: https://pure.mpg.de/cone/journals/resource/954927546241