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  Role of the regulatory genes SEF1, VMA1 and SFU1 in riboflavin synthesis in the flavinogenic yeast Candida famata (Candida flareri)

Andreieva, Y., Petroyska, Y., Lyzak, O., Liu, W., Kang, Y., Dmytruk, K., et al. (2020). Role of the regulatory genes SEF1, VMA1 and SFU1 in riboflavin synthesis in the flavinogenic yeast Candida famata (Candida flareri). Yeast, 37(9-10, SI: ISSY35), 497-504. doi:10.1002/yea.3503.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-B9B0-2 Version Permalink: http://hdl.handle.net/21.11116/0000-0007-4CEB-C
Genre: Journal Article

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 Creators:
Andreieva, Yuliia1, Author
Petroyska, Yana1, Author
Lyzak, Oleksii2, Author              
Liu, Wen1, Author
Kang, Yingqian1, Author
Dmytruk, Kostyantyn1, Author
Sibirny, Andriy1, Author
Affiliations:
1external, ou_persistent22              
2Zachariae, Wolfgang / Chromosome Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565176              

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Free keywords: FAMATA DEBARYOMYCES-HANSENII; CANDIDA-FAMATA; D-FABRYI; TRANSFORMATION; CONSTRUCTION; TROPICALIS; GENECandida famata; riboflavin; SEF1; SFU1; VMA1; yeast;
 Abstract: Riboflavin or vitamin B(2)is an essential dietary component for humans and animals that is the precursor of flavin coenzymes flavin mononucleotide and flavin adenine dinucleotide involved in numerous enzymatic reactions. The flavinogenic yeastCandida famataoverproduces riboflavin under iron starvation; however, regulation of this process is poorly understood. Regulatory geneSEF1encoding transcription activator has been identified. Its deletion blocks yeast ability to overproduce riboflavin under iron starvation. It was shown here that theSEF1promoters from other flavinogenic (Candida albicans) and non-flavinogenic (Candida tropicalis) yeasts fused with the open reading frame (ORF) ofSEF1gene fromC. famataare able to restore riboflavin oversynthesis insef1 Delta mutants. It is known that in the pathogenic flavinogenic yeastC. albicans, Sfu1 (GATA-type transcription factor) repressesSEF1.Here, we found that deletion ofSFU1gene in wild-typeC. famataleads to riboflavin oversynthesis. Moreover, it was shown that disruption ofVMA1gene (coding for vacuolar ATPase subunit A) also results in riboflavin oversynthesis inC. famata.

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Language(s): eng - English
 Dates: 2020-062020-10
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000544911100001
DOI: 10.1002/yea.3503
 Degree: -

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Title: Yeast
Source Genre: Journal
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Publ. Info: New York, NY : John Wiley & Sons
Pages: - Volume / Issue: 37 (9-10, SI: ISSY35) Sequence Number: - Start / End Page: 497 - 504 Identifier: ISSN: 0749-503X
CoNE: https://pure.mpg.de/cone/journals/resource/110990112651288_1