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  Long-term live-cell imaging techniques for visualizing pavement cell morphogenesis

Seerangan, K., van Spoordonk, R., Sampathkumar, A., & Eng, R. (2020). Long-term live-cell imaging techniques for visualizing pavement cell morphogenesis. In Methods in cell biology (pp. 365-380).

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Item Permalink: http://hdl.handle.net/21.11116/0000-0007-0054-A Version Permalink: http://hdl.handle.net/21.11116/0000-0007-0055-9
Genre: Book Chapter

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 Creators:
Seerangan, K.1, Author              
van Spoordonk, R.1, Author              
Sampathkumar, A.1, Author              
Eng, R.C.1, Author              
Affiliations:
1Plant Cell Biology and Microscopy, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2253647              

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Free keywords: Cotyledon, Leaf, Pavement cells, Microtubules, Live-cell imaging, Microscopy, Morphogenesis, Cell shape, Cell growth, Arabidopsis
 Abstract: Recent advancements in microscopy and biological technologies have allowed scientists to study dynamic plant developmental processes with high temporal and spatial resolution. Pavement cells, epidermal cells found on leaf tissue, form complex shapes with alternating regions of indentations and outgrowths that are postulated to be driven by the microtubule cytoskeleton. Given their complex shapes, pavement cells and the microtubule contribution towards morphogenesis have been of great interest in the field of developmental biology. Here, we focus on two live-cell imaging methods that allow for early and long-term imaging of the cotyledon (embryonic leaf-like tissue) and leaf epidermis with minimal invasiveness in order to study microtubules throughout pavement cell morphogenesis. The methods described in this chapter can be applied to studying other developmental processes associated with cotyledon and leaf tissue.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: Methods in cell biology
Source Genre: Book
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Publ. Info: -
Pages: - Volume / Issue: 160 Sequence Number: - Start / End Page: 365 - 380 Identifier: -

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Title: Plant Cell Biology
Source Genre: Book
 Creator(s):
Anderson, Charles T., Editor
Haswell, Elizabeth S., Editor
Dixit, Ram, Editor
Affiliations:
-
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: -