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  Automated glycan assembly of plant cell wall oligosaccharides

Pfrengle, F. (2020). Automated glycan assembly of plant cell wall oligosaccharides. Methods in Molecular Biology, 2149, 503 -512. doi:10.1007/978-1-0716-0621-6_28.

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Article.pdf (Publisher version), 586KB
 
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 Creators:
Pfrengle, Fabian1, Author              
Affiliations:
1Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863303              

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Free keywords: Plant cell wall oligosaccharides, Automated glycan assembly, Solid-phase synthesis, Arabinoxylan, Arabinogalactan, Xyloglucan
 Abstract: Synthetic cell wall oligosaccharides are promising molecular tools for investigating the structure and function of plant cell walls. Their well-defined structure and high purity prevents misinterpretations of experimental data, and the possibility to introduce chemical handles provides means for easier localization and detection. Automated glycan assembly as emerged has a powerful new method for the efficient preparation of oligosaccharide libraries. We recently made use of this technology to prepare a collection of plant cell wall glycans for cell wall research. In this chapter, detailed experimental procedures for the automated synthesis of oligosaccharides that are ready for use in biological assays are described.

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Language(s): eng - English
 Dates: 2020-07-032020
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-0716-0621-6_28
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Title: Methods in Molecular Biology
  Other : Methods Mol. Biol.
Source Genre: Journal
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Publ. Info: US : Springer
Pages: - Volume / Issue: 2149 Sequence Number: - Start / End Page: 503 - 512 Identifier: ISSN: 1064-3745

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Title: The plant cell wall
Source Genre: Book
 Creator(s):
Popper, Zoe, Editor
Affiliations:
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Publ. Info: New York, NY : Humana
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISBN: 978-1-0716-0621-6