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  Unilateral Cleavage Furrows in Multinucleate Cells

Bindl, J., Molnar, E. S., Ecke, M., Prassler, J., Mueller-Taubenberger, A., & Gerisch, G. (2020). Unilateral Cleavage Furrows in Multinucleate Cells. CELLS, 9(6): 1493. doi:10.3390/cells9061493.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-C9F1-7 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-C9F2-6
Genre: Journal Article

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 Creators:
Bindl, Julia1, Author              
Molnar, Eszter Sarolta1, Author              
Ecke, Mary1, Author              
Prassler, Jana1, Author              
Mueller-Taubenberger, Annette2, Author
Gerisch, Günther1, Author              
Affiliations:
1Gerisch, Günther / Cell Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565149              
2external, ou_persistent22              

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Free keywords: HEAVY-CHAIN GENE; MYOSIN-II; CONTRACTILE RING; DICTYOSTELIUM; ACTIN; CYTOKINESIS; MEMBRANE; CORTEXILLIN; RECRUITMENT; ANILLINcell fusion; cortexillin; cytokinesis; Dictyostelium; myosin;
 Abstract: Multinucleate cells can be produced inDictyosteliumby electric pulse-induced fusion. In these cells, unilateral cleavage furrows are formed at spaces between areas that are controlled by aster microtubules. A peculiarity of unilateral cleavage furrows is their propensity to join laterally with other furrows into rings to form constrictions. This means cytokinesis is biphasic in multinucleate cells, the final abscission of daughter cells being independent of the initial direction of furrow progression. Myosin-II and the actin filament cross-linking protein cortexillin accumulate in unilateral furrows, as they do in the normal cleavage furrows of mononucleate cells. In a myosin-II-null background, multinucleate or mononucleate cells were produced by cultivation either in suspension or on an adhesive substrate. Myosin-II is not essential for cytokinesis either in mononucleate or in multinucleate cells but stabilizes and confines the position of the cleavage furrows. In fused wild-type cells, unilateral furrows ingress with an average velocity of 1.7 mu m x min(-1), with no appreciable decrease of velocity in the course of ingression. In multinucleate myosin-II-null cells, some of the furrows stop growing, thus leaving space for the extensive broadening of the few remaining furrows.

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Language(s): eng - English
 Dates: 2020
 Publication Status: Published online
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: ISI: 000550039200001
DOI: 10.3390/cells9061493
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Title: CELLS
Source Genre: Journal
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Publ. Info: ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND : MDPI
Pages: - Volume / Issue: 9 (6) Sequence Number: 1493 Start / End Page: - Identifier: -