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Schlagwörter:
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Zusammenfassung:
Reaction centres of photosynthesis isolated from the photosynthetic bacterium Rhodopseudomonas viridis by a single step of molecular sieve chromatography were crystallized. The isolated and the crystallized reaction centres contain four different protein subunits, including a membrane-bound cytochrome. Crystallization was achieved by salt precipitation using 2 to 3 m-ammonium sulphate as a precipitating agent in the presence of N,N-dimethyldodecylamine N-oxide as detergent and heptane-1,2,3-triol. Large tetragonal crystals of space group P41212 or its enantiomorph diffracting to beyond 2.5 Å were obtained.
