English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Seeing natural images through the eye of a fly with remote focusing two-photon microscopy

Schuetzenberger, A., & Borst, A. (2020). Seeing natural images through the eye of a fly with remote focusing two-photon microscopy. iScience, 23(6): 101170. doi:10.1016/j.isci.2020.101170.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
1-s2.0-S2589004220303552-main.pdf (Publisher version), 7MB
Name:
1-s2.0-S2589004220303552-main.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
open access article
:
1-s2.0-S2589004220303552-mmc1.pdf (Supplementary material), 4MB
Name:
1-s2.0-S2589004220303552-mmc1.pdf
Description:
Data and Code Availability The modified version of ScanImage 5.1 created in this study is available at GitHub (https://github.com/borstlab/Scanimage_Planes3D). The datasets and the data analysis code generated during this study is available at GitHub (https://github.com/borstlab/obique-planes).
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Schuetzenberger, Anna1, Author           
Borst, Alexander1, Author           
Affiliations:
1Department: Circuits-Computation-Models / Borst, MPI of Neurobiology, Max Planck Society, ou_1113548              

Content

show
hide
Free keywords: RECEPTIVE-FIELDS; LIGHT ADAPTATION; VISUAL-SYSTEM; DROSOPHILA; RETINA; CIRCUITRY; SIGNALS; INPUT; PATHWAYS; DYNAMICS
 Abstract: Visual systems of many animals, including the fruit fly Drosophila, represent the surrounding space as 2D maps, formed by populations of neurons. Advanced genetic tools make the fly visual system especially well accessible. However, in typical in vivo preparations for two-photon calcium imaging, relatively few neurons can be recorded at the same time. Here, we present an extension to a conventional two-photon microscope, based on remote focusing, which enables real-time rotation of the imaging plane, and thus flexible alignment to cellular structures, without resolution or speed trade-off. We simultaneously record from over 100 neighboring cells spanning the 2D retinotopic map. We characterize its representation of moving natural images, which we find is comparable to noise predictions. Our method increases throughput 10-fold and allows us to visualize a significant fraction of the fly's visual field. Furthermore, our system can be applied in general for a more flexible investigation of neural circuits.

Details

show
hide
Language(s): eng - English
 Dates: 2020-06-26
 Publication Status: Published in print
 Pages: 29
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: iScience
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam ; Bosten ; London ; New York ; Oxford ; Paris ; Philadelphia ; San Diego ; St. Louis : Elsevier
Pages: - Volume / Issue: 23 (6) Sequence Number: 101170 Start / End Page: - Identifier: ISSN: 2589-0042
CoNE: https://pure.mpg.de/cone/journals/resource/2589-0042