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  Chimeric oligosaccharide conjugate induces opsonic antibodies against Streptococcus pneumoniae serotypes 19A and 19F

Sanapala, S. R., Silva Seco, B. M., Baek, J. Y., Awan, S. I., Pereira, C. L., & Seeberger, P. H. (2020). Chimeric oligosaccharide conjugate induces opsonic antibodies against Streptococcus pneumoniae serotypes 19A and 19F. Chemical Science, 11(28), 7401-7407. doi:10.1039/D0SC02230F.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-CF90-E Version Permalink: http://hdl.handle.net/21.11116/0000-0006-CF91-D
Genre: Journal Article

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 Creators:
Sanapala , Someswara Rao1, Author
Silva Seco, Bruna Mara1, Author              
Baek, Ju Yuel1, Author              
Awan , Shahid I.1, Author
Pereira, Claney L., Author
Seeberger, Peter H.1, Author              
Affiliations:
1Peter H. Seeberger - Vaccine Development, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863308              

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 Abstract: Streptococcus pneumoniae 19A (ST19A) and 19F (ST19F) are among the prevalent serotypes causing pneumococcal disease worldwide even after introduction of a 13-valent pneumococcal conjugate vaccine (PCV13). Synthetic glycoconjugate vaccines have defined chemical structures rather than isolated polysaccharide mixtures utilized in marketed vaccines. Ideally, a minimal number of synthetic antigens would cover as many bacterial serotypes to lower cost of goods and minimize the response to carrier proteins. To demonstrate that a chimeric oligosaccharide antigen can induce a protective immune response against multiple serotypes, we synthesized a chimeric antigen (ST19AF) that is comprised of a repeating unit of ST19A and ST19F capsular polysaccharide each. Synthetic glycan epitopes representing only ST19A, and ST19F were prepared for comparison. Semisynthetic glycoconjugates containing chimeric antigen ST19AF induced high antibody titers able to recognize native CPS from ST19A and ST19F in rabbits. The antibodies were able to kill both strains of pneumococci. Chimeric antigens are an attractive means to induce an immune response against multiple bacterial serotypes.

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Language(s): eng - English
 Dates: 2020-06-262020
 Publication Status: Published in print
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 Identifiers: DOI: 10.1039/D0SC02230F
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Title: Chemical Science
  Other : Chem. Sci.
Source Genre: Journal
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Publ. Info: Cambridge, UK : Royal Society of Chemistry
Pages: - Volume / Issue: 11 (28) Sequence Number: - Start / End Page: 7401 - 7407 Identifier: ISSN: 2041-6520