English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  In-cell architecture of an actively transcribing-translating expressome

O’Reilly, F. J., Xue, L., Graziadei, A., Sinn, L., Lenz, S., Tegunov, D., et al. (2020). In-cell architecture of an actively transcribing-translating expressome. Science, 369(6503), 554-557. doi:10.1126/science.abb3758.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
3247325.pdf (Publisher version), 5MB
 
File Permalink:
-
Name:
3247325.pdf
Description:
-
Visibility:
Restricted ( Max Planck Society (every institute); )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3247325-Suppl-1.pdf (Supplementary material), 8MB
Name:
3247325-Suppl-1.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3247325-Suppl-2.xlsx (Supplementary material), 917KB
Name:
3247325-Suppl-2.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3247325-Suppl-3.xlsx (Supplementary material), 690KB
Name:
3247325-Suppl-3.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3247325-Suppl-4.xlsx (Supplementary material), 15KB
Name:
3247325-Suppl-4.xlsx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3247325-Suppl-5.pdf (Supplementary material), 109KB
Name:
3247325-Suppl-5.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3247325-Suppl-6.mp4 (Supplementary material), 12MB
Name:
3247325-Suppl-6.mp4
Description:
-
Visibility:
Public
MIME-Type / Checksum:
video/mp4 / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
3247325-Suppl-7.mp4 (Supplementary material), 12MB
Name:
3247325-Suppl-7.mp4
Description:
-
Visibility:
Public
MIME-Type / Checksum:
video/mp4 / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
O’Reilly, F. J., Author
Xue, L., Author
Graziadei, A., Author
Sinn, L., Author
Lenz, S., Author
Tegunov, D.1, Author              
Blötz, C., Author
Singh, N., Author
Hagen, W. J. H., Author
Cramer, P.1, Author              
Stülke, J., Author
Mahamid, J., Author
Rappsilber, J., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

Content

show
hide
Free keywords: -
 Abstract: Structural biology studies performed inside cells can capture molecular machines in action within their native context. In this work, we developed an integrative in-cell structural approach using the genome-reduced human pathogen Mycoplasma pneumoniae. We combined whole-cell cross-linking mass spectrometry, cellular cryo–electron tomography, and integrative modeling to determine an in-cell architecture of a transcribing and translating expressome at subnanometer resolution. The expressome comprises RNA polymerase (RNAP), the ribosome, and the transcription elongation factors NusG and NusA. We pinpointed NusA at the interface between a NusG-bound elongating RNAP and the ribosome and propose that it can mediate transcription-translation coupling. Translation inhibition dissociated the expressome, whereas transcription inhibition stalled and rearranged it. Thus, the active expressome architecture requires both translation and transcription elongation within the cell.

Details

show
hide
Language(s): eng - English
 Dates: 2020-07-312020-07-31
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/science.abb3758
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Science
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 369 (6503) Sequence Number: - Start / End Page: 554 - 557 Identifier: -