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  Environmentally sensitive color‐shifting fluorophores for bioimaging

Wang, L., Hiblot, J., Popp, C., Xue, L., & Johnsson, K. (2020). Environmentally sensitive color‐shifting fluorophores for bioimaging. Angewandte Chemie International Edition, 59(49), 21880-21884. doi:10.1002/anie.202008357.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-D628-C Version Permalink: http://hdl.handle.net/21.11116/0000-0007-A1DB-C
Genre: Journal Article

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AngewChemIntEd_59_2020_21880.pdf (Any fulltext), 2MB
 
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AngewChemIntEd_59_2020_21880_Suppl.pdf (Supplementary material), 10MB
 
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 Creators:
Wang, Lu1, Author              
Hiblot, Julien1, Author              
Popp, Christoph1, Author              
Xue, Lin1, Author              
Johnsson, Kai1, Author              
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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Free keywords: color-shifting • bioimaging • fluorescence • sensor • ratiometric
 Abstract: We introduce color‐shifting fluorophores which reversibly switch between a green and red fluorescent form via an intramolecular spirocyclization. The equilibrium of spirocyclization is environmentally sensitive and can be directly measured by determining the ratio of red to green fluorescence, allowing the generation of ratiometric fluorescent probes and biosensors. Specifically, we develop a ratiometric biosensor for imaging calcium ions (Ca 2+ ) in living cells, ratiometric probes for different proteins and a bioassay for the quantification of nicotinamide adenine dinucleotide phosphate.

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Language(s): eng - English
 Dates: 2020-06-122020-08-062020-09-282020-12-01
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/anie.202008357
 Degree: -

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Title: Angewandte Chemie International Edition
  Abbreviation : Angew. Chem., Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 59 (49) Sequence Number: - Start / End Page: 21880 - 21884 Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851