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  Channelrhodopsin-mediated optogenetics highlights a central role of depolarization-dependent plant proton pumps

Reyer, A., Häßler, M., Scherzer, S., Huang, S., Pedersen, J. T., Al-Rascheid, K. A. S., et al. (2020). Channelrhodopsin-mediated optogenetics highlights a central role of depolarization-dependent plant proton pumps. Proceedings of the National Academy of Sciences of the United States of America. doi:10.1073/pnas.2005626117.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-D983-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-D989-B
Genre: Journal Article


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Reyer, Antonella1, Author
Häßler, Melanie1, Author
Scherzer, Sönke1, Author
Huang, Shouguang1, Author
Pedersen, Jesper Torbøl2, Author
Al-Rascheid, Khaled A. S.3, Author
Bamberg, Ernst4, Author              
Palmgren, Michael2, Author
Dreyer, Ingo5, Author
Nagel, Georg1, Author
Hedrich, Rainer1, Author
Becker, Dirk1, Author
1Institute for Molecular Plant Physiology and Biophysics, University of Würzburg, Würzburg, Germany, ou_persistent22              
2Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg C, Denmark, ou_persistent22              
3Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia, ou_persistent22              
4Emeritusgroup Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2253652              
5Center of Bioinformatics, Simulation and Modeling, Faculty of Engineering, Universidad de Talca, Talca, Chile, ou_persistent22              


Free keywords: Arabidopsis, electrical signaling, optogenetics, PM H+-ATPase
 Abstract: In plants, environmental stressors trigger plasma membrane depolarizations. Being electrically interconnected via plasmodesmata, proper functional dissection of electrical signaling by electrophysiology is basically impossible. The green alga Chlamydomonas reinhardtii evolved blue light-excited channelrhodopsins (ChR1, 2) to navigate. When expressed in excitable nerve and muscle cells, ChRs can be used to control the membrane potential via illumination. In Arabidopsis plants, we used the algal ChR2-light switches as tools to stimulate plasmodesmata-interconnected photosynthetic cell networks by blue light and monitor the subsequent plasma membrane electrical responses. Blue-dependent stimulations of ChR2 expressing mesophyll cells, resting around -160 to -180 mV, reproducibly depolarized the membrane potential by 95 mV on average. Following excitation, mesophyll cells recovered their prestimulus potential not without transiently passing a hyperpolarization state. By combining optogenetics with voltage-sensing microelectrodes, we demonstrate that plant plasma membrane AHA-type H+-ATPase governs the gross repolarization process. AHA2 protein biochemistry and functional expression analysis in Xenopus oocytes indicates that the capacity of this H+ pump to recharge the membrane potential is rooted in its voltage- and pH-dependent functional anatomy. Thus, ChR2 optogenetics appears well suited to noninvasively expose plant cells to signal specific depolarization signatures. From the responses we learn about the molecular processes, plants employ to channel stress-associated membrane excitations into physiological responses.


Language(s): eng - English
 Dates: 2020-03-252020-07-022020-08-11
 Publication Status: Published online
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1073/pnas.2005626117
BibTex Citekey: reyer_channelrhodopsin-mediated_2020
 Degree: -



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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Acad. Sci. USA
  Other : Proc. Acad. Sci. U.S.A.
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : PNAS
Source Genre: Journal
Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230