English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Multicolor 3D MINFLUX nanoscopy of mitochondrial MICOS proteins

Pape, J. K., Stephan, T., Balzarotti, F., Büchner, R., Lange, F., Riedel, D., et al. (2020). Multicolor 3D MINFLUX nanoscopy of mitochondrial MICOS proteins. Proceedings of the National Academy of Sciences of the United States of America, 1-8. doi:10.1073/pnas.2009364117.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/21.11116/0000-0006-DAF7-E Version Permalink: http://hdl.handle.net/21.11116/0000-0006-DAF8-D
Genre: Journal Article

Files

show Files
hide Files
:
PNAS_epub_2020_117.pdf (Any fulltext), 4MB
 
File Permalink:
-
Name:
PNAS_epub_2020_117.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
PNAS_epub_2020_117_Suppl.pdf (Supplementary material), 3MB
 
File Permalink:
-
Name:
PNAS_epub_2020_117_Suppl.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Description:
-
Locator:
https://doi.org/10.1073/pnas.2009364117 (Any fulltext)
Description:
-

Creators

show
hide
 Creators:
Pape, Jasmin K., Author
Stephan, Till, Author
Balzarotti, Francisco, Author
Büchner, Rebecca, Author
Lange, Felix, Author
Riedel, Dietmar, Author
Jakobs, Stefan, Author
Hell, Stefan W.1, Author              
Affiliations:
1Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

Content

show
hide
Free keywords: MINFLUX | superresolution microscopy | mitochondria | MICOS | cluster analysis
 Abstract: The mitochondrial contact site and cristae organizing system (MICOS) is a multisubunit protein complex that is essential for the proper architecture of the mitochondrial inner membrane. MICOS plays a key role in establishing and maintaining crista junctions, tubular or slit-like structures that connect the cristae membrane with the inner boundary membrane, thereby ensuring a contiguous inner membrane. MICOS is enriched at crista junctions, but the detailed distribution of its subunits around crista junctions is unclear because such small length scales are inaccessible with established fluorescence microscopy. By targeting individually activated fluorophores with an excitation beam featuring a central zero-intensity point, the nanoscopy method called MINFLUX delivers single-digit nanometer-scale three-dimensional (3D) resolution and localization precision. We employed MINFLUX nanoscopy to investigate the submitochondrial localization of the core MICOS subunit Mic60 in relation to two other MICOS proteins, Mic10 and Mic19. We demonstrate that dual-color 3D MINFLUX nanoscopy is applicable to the imaging of organellar substructures, yielding a 3D localization precision of ∼5 nm in human mitochondria. This isotropic precision facilitated the development of an analysis framework that assigns localization clouds to individual molecules, thus eliminating a source of bias when drawing quantitative conclusions from single-molecule localization microscopy data. MINFLUX recordings of Mic60 indicate ringlike arrangements of multiple molecules with a diameter of 40 to 50 nm, suggesting that Mic60 surrounds individual crista junctions. Statistical analysis of dual-color MINFLUX images demonstrates that Mic19 is generally in close proximity to Mic60, whereas the spatial coordination of Mic10 with Mic60 is less regular, suggesting structural heterogeneity of MICOS.

Details

show
hide
Language(s): eng - English
 Dates: 2020-06-232020
 Publication Status: Published online
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1073/pnas.2009364117
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : Proc. Acad. Sci. USA
  Other : Proc. Acad. Sci. U.S.A.
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : PNAS
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 1 - 8 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230