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  Serial femtosecond and serial synchrotron crystallography yield data of equivalent quality: a systematic comparison

Mehrabi, P., Bücker, R., Bourenkov, G., Ginn, H., von Stetten, D., Mueller-Werkmeister, H. M., et al. (2020). Serial femtosecond and serial synchrotron crystallography yield data of equivalent quality: a systematic comparison. doi:10.1101/2020.08.21.257170.

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Item Permalink: http://hdl.handle.net/21.11116/0000-0006-E333-0 Version Permalink: http://hdl.handle.net/21.11116/0000-0006-E334-F
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2020.08.21.257170v1.full.pdf (Preprint), 4MB
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2020.08.21.257170v1.full.pdf
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 Creators:
Mehrabi, P.1, 2, 3, 4, Author              
Bücker, R.1, Author              
Bourenkov, G.5, Author
Ginn, H.6, Author
von Stetten, D.5, Author
Mueller-Werkmeister, H. M.6, Author
Kuo, A.7, Author
Morizumi, T.7, Author
Eger, B.7, Author
Ou, W.-L.7, Author
Oghbaey, S.8, Author
Sarracini, A.8, Author
Besaw, J.7, 8, Author
Paré-Labrosse, O.1, 8, Author              
Meier, S.9, Author
Schikora, H.10, Author              
Tellkamp, F.10, Author              
Marx, A.1, Author              
Sherrell, D. A.6, 11, Author
Axford, D.6, Author
Owen, R. L.6, AuthorErnst, O. P.7, 12, AuthorPai, Emil F.2, 4, 7, AuthorSchulz, E.-C.1, 4, Author              Miller, R. J. D.1, 4, 8, 9, Author               more..
Affiliations:
1Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
2Department of Medical Biophysics, University of Toronto, ou_persistent22              
3The Campbell Family Cancer Research Institute, Ontario, Cancer Institute, ou_persistent22              
4Center for Free-Electron Laser Science, ou_persistent22              
5European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen Synchrotron (DESY), ou_persistent22              
6Diamond Light Source, Harwell Science and Innovation Campus, ou_persistent22              
7Department of Biochemistry, University of Toronto, ou_persistent22              
8Departments of Chemistry and Physics, University of Toronto, ou_persistent22              
9Department of Physics, Universität Hamburg, ou_persistent22              
10Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2074322              
11Structural BiologyCenter, X-ray Science Division, Argonne National Laboratory, ou_persistent22              
12Department of Molecular Genetics, University of Toronto, ou_persistent22              

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 Abstract: For the two proteins myoglobin (MB) and fluoroacetate dehalogenase (FAcD), we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maximize comparability, we used the same batch of crystals, the same sample delivery device, as well as the same data analysis software. Overall figures of merit indicate that the data of both radiation sources are of equivalent quality. For both proteins reasonable data statistics can be obtained with approximately 5000 room temperature diffraction images irrespective of the radiation source. The direct comparability of SSX and SFX data indicates that diffraction quality is rather linked to the properties of the crystals than to the radiation source. Time-resolved experiments can therefore be conducted at the source that best matches the desired time-resolution.

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Language(s): eng - English
 Dates: 2020-08-22
 Publication Status: Published online
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: No review
 Identifiers: DOI: 10.1101/2020.08.21.257170
 Degree: -

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