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  Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison

Mehrabi, P., Bücker, R., Bourenkov, G., Ginn, H., von Stetten, D., Mueller-Werkmeister, H. M., et al. (2021). Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison. Science Advances, 7(12): eabf1380. doi:10.1126/sciadv.abf1380.

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eabf1380.full-1.pdf (Publisher version), 607KB
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This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
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2021
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© The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

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https://dx.doi.org/10.1126/sciadv.abf1380 (Publisher version)
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 Creators:
Mehrabi, P.1, 2, 3, 4, Author              
Bücker, R.1, 4, 5, Author              
Bourenkov, G.6, Author
Ginn, H.7, Author
von Stetten, D.6, Author
Mueller-Werkmeister, H. M.8, Author
Kuo, A.9, Author
Morizumi, T.9, Author
Eger, B.9, Author
Ou, W.-L.9, Author
Oghbaey, S.10, Author
Sarracini, A.10, Author
Besaw, J.9, 10, Author
Paré-Labrosse, O.1, 4, 10, Author              
Meier, S.11, Author
Schikora, H.4, 12, Author              
Tellkamp, F.4, 12, Author              
Marx, A.1, 4, Author              
Sherrell, D. A.7, 13, Author
Axford, D.7, Author
Owen, R. L.7, AuthorErnst, O. P.9, 14, AuthorPai, Emil F.2, 3, 9, AuthorSchulz, E.-C.1, 4, Author              Miller, R. J. D.1, 4, 10, 11, Author               more..
Affiliations:
1Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
2Department of Medical Biophysics, University of Toronto, ou_persistent22              
3The Campbell Family Cancer Research Institute, Ontario, Cancer Institute, ou_persistent22              
4Center for Free-Electron Laser Science, ou_persistent22              
5Centre for Structural Systems Biology, Department of Chemistry, University of Hamburg, ou_persistent22              
6European Molecular Biology Laboratory (EMBL), Hamburg Outstation c/o Deutsches Elektronen Synchrotron (DESY), ou_persistent22              
7Diamond Light Source, Harwell Science and Innovation Campus, ou_persistent22              
8Institute of Chemistry–Physical Chemistry, University of Potsdam, ou_persistent22              
9Department of Biochemistry, University of Toronto, ou_persistent22              
10Departments of Chemistry and Physics, University of Toronto, ou_persistent22              
11Department of Physics, Universität Hamburg, ou_persistent22              
12Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2074322              
13Structural BiologyCenter, X-ray Science Division, Argonne National Laboratory, ou_persistent22              
14Department of Molecular Genetics, University of Toronto, ou_persistent22              

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 Abstract: For the two proteins myoglobin and fluoroacetate dehalogenase, we present a systematic comparison of crystallographic diffraction data collected by serial femtosecond (SFX) and serial synchrotron crystallography (SSX). To maximize comparability, we used the same batch of micron-sized crystals, the same sample delivery device, and the same data analysis software. Overall figures of merit indicate that the data of both radiation sources are of equivalent quality. For both proteins, reasonable data statistics can be obtained with approximately 5000 room-temperature diffraction images irrespective of the radiation source. The direct comparability of SSX and SFX data indicates that the quality of diffraction data obtained from these samples is linked to the properties of the crystals rather than to the radiation source. Therefore, for other systems with similar properties, time-resolved experiments can be conducted at the radiation source that best matches the desired time resolution.

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Language(s): eng - English
 Dates: 2020-10-072021-01-282021-03-17
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Grant ID : 623994
Funding program : Funding Programme 7 (FP7)
Funding organization : European Commission (EC)

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Title: Science Advances
  Other : Sci. Adv.
Source Genre: Journal
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Publ. Info: Washington : AAAS
Pages: - Volume / Issue: 7 (12) Sequence Number: eabf1380 Start / End Page: - Identifier: ISSN: 2375-2548
CoNE: https://pure.mpg.de/cone/journals/resource/2375-2548