Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
 
 
DownloadE-Mail
 Lokale TagsFreigabegeschichteDetailsÜbersicht
  A Step-by-Step Protocol for COMPASS, a Synthetic Biology Tool for Combinatorial Gene Assembly

Naseri, G., & Mueller-Roeber, B. (2020). A Step-by-Step Protocol for COMPASS, a Synthetic Biology Tool for Combinatorial Gene Assembly. In S. Chandran, & K. W. George (Eds.), DNA Cloning and Assembly: Methods and Protocols (pp. 277-303). New York, NY: Springer US.

Item is

 

einblenden: alle ausblenden: alle

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: https://hdl.handle.net/21.11116/0000-0006-E58E-8 Versions-Permalink: https://hdl.handle.net/21.11116/0000-0006-E58F-7
Genre: Buchkapitel

Externe Referenzen

einblenden:
ausblenden:
externe Referenz:
Link (beliebiger Volltext)
Beschreibung:
-
OA-Status:

Urheber

einblenden:
ausblenden:
 Urheber:
Naseri, Gita1, Autor
Mueller-Roeber, B.2, Autor           
Affiliations:
1External Organizations, ou_persistent22              
2Plant Signalling, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753311              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: For industry-scale production of high-value chemicals in microbial cell factories, the elimination of metabolic flux imbalances is a critical aspect. However, a priori knowledge about the genetic design of optimal production pathways is typically not available. COMPASS, COMbinatorial Pathway ASSembly, is a rapid cloning method for the balanced expression of multiple genes in biochemical pathways. The method generates thousands of individual DNA constructs in modular, parallel, and high-throughput manner. COMPASS employs inducible artificial transcription factors derived from plant (Arabidopsis thaliana) regulators to control the expression of pathway genes in yeast (Saccharomyces cerevisiae). It utilizes homologous recombination for parts assembly and employs a positive selection scheme to identify correctly assembled pathway variants after both in vivo and in vitro recombination. Finally, COMPASS is equipped with a CRISPR/Cas9 genome modification system allowing for the one-step multilocus integration of genes. Although COMPASS was initially developed for pathway engineering, it can equally be employed for balancing gene expression in other synthetic biology projects.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2020
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: DOI: 10.1007/978-1-0716-0908-8_16
BibTex Citekey: Naseri2020
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: DNA Cloning and Assembly: Methods and Protocols
Genre der Quelle: Buch
 Urheber:
Chandran, Sunil, Herausgeber
George, Kevin W., Herausgeber
Affiliations:
-
Ort, Verlag, Ausgabe: New York, NY : Springer US
Seiten: - Band / Heft: - Artikelnummer: - Start- / Endseite: 277 - 303 Identifikator: ISBN: 978-1-0716-0908-8